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Fungal inhibitory lactic acid bacteria

Characterization and application of Lactobacillus plantarum MiLAB 393

Ström, Katrin (2005). Fungal inhibitory lactic acid bacteria. Diss. (sammanfattning/summary) Uppsala : Sveriges lantbruksuniv., Acta Universitatis Agriculturae Sueciae, 1652-6880 ; 2005:37
ISBN 91-576-7036-6
[Doctoral thesis]

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Lactic acid bacteria (LAB) are microorganisms that have been used for centuries to prepare and improve storage of food and for ensiling of different crops for animal feed. This thesis explores the possibility of using LAB to inhibit growth of spoilage fungi in food and feed products. LAB isolates, collected from plant material or dairy products, were screened for antifungal activity in a dual culture assay. Strains with antifungal activity were identified and the fungal inhibitory activity was further evaluated. The majority of the identified isolates belonged to the genus Lactobacillus and L. coryniformis was the dominating species. A bioassay-guided isolation method was developed to detect active compounds and Aspergillus fumigatus and Penicillium commune were used as indicator organisms. We identified two cyclic dipeptides, cyclo(L-Phe-L-Pro) and cyclo(L-Phe-trans-4-OH-L-Pro), and 3-phenyl lactic acid as fungal inhibitory compounds from L. plantarum MiLAB 393 and L. coryniformis Si3. Analysis of cell free supernatants indicate that these compounds are also produced by a Pediococcus pentosaceus and a L. sakei strain, together with yet another L. plantarum isolate. One strain, Lactobacillus plantarum L4, also produced the hydroxylated fatty acids 3-hydroxy dodecanoic acid and 3-hydroxy-5-cis-dodecenoic acid. Co-cultivation of L. plantarum MiLAB 393 and Aspergillus nidulans revealed that the fungus was affected both by morphological changes and that the total mass of mycelia was clearly decreased. Protein extracts were prepared from co-cultivated mycelia and changes in protein abundance were detected using two-dimensional differential display. Selected isolates were evaluated for their potential to enhance aerobic storage stability of grass silage. Four LAB strains improved aerobic storage stability and one strain, L. plantarum MiLAB 393, inhibited growth of spoilage yeast effectively. The actual mode of action of the identified compounds in this study has not yet been established. However, it is important to consider that these substances probably act synergistically and that other metabolic products, especially lactic acid, also contributes to the antifungal activity.

Authors/Creators:Ström, Katrin
Title:Fungal inhibitory lactic acid bacteria
Subtitle:Characterization and application of Lactobacillus plantarum MiLAB 393
Series Name/Journal:Acta Universitatis Agriculturae Sueciae
Year of publishing :2005
Number of Pages:38
ALLI. Magnusson, J., Ström, K., Roos, S., Sjögren, J. & Schnürer, J. 2003. Broad and complex antifungal activity among environmental isolates of lactic acid bacteria. FEMS Microbiology Letters 219, 129-135. II. Ström, K., Sjögren, J., Broberg, A. & Schnürer, J. 2002. Lactobacillus plantarum MiLAB 393 produces the antifungal cyclic dipeptides cyclo(L-Phe-L-Pro) and cyclo(L-Phe-trans-4-OH-L-Pro) and 3-phenyllactic acid. Applied and Environmental Microbiology 68, 4322-4327. III. Ström, K., Schnürer, J & Melin, P. 2005. Co-cultivation of antifungal Lactobacillus plantarum MiLAB 393 and Aspergillus nidulans, evaluation of effects on fungal growth and protein expression. FEMS Microbiology Letters. (In press). IV. Ström, K., Pauli, T., Ohlsson, C. & Schnürer, J. Lactic Acid Bacteria with Antifungal Properties Can Provide Aerobic Stability and Improve the Quality of Grass Silage. (Manuscript).
Place of Publication:Uppsala
ISBN for printed version:91-576-7036-6
Publication Type:Doctoral thesis
Full Text Status:Public
Agris subject categories.:Q Food science > Q52 Feed processing and preservation
Q Food science > Q53 Feed contamination and toxicology
Subjects:Not in use, please see Agris categories
Agrovoc terms:lactic acid bacteria, lactobacillus plantarum, antifungal properties, moulds, yeasts, silage making, deterioration, preservation
Keywords:antifungal, mould, mold, yeast, Aspergillus nidulans, aerobic stability, silage inoculant, 3-hydroxy fatty acids, 2, 5-diketopiperazines, cyclic dipeptides, phenyllactic acid
Permanent URL:
ID Code:802
Department:(NL, NJ) > Dept. of Microbiology (until 161231)
Deposited By: Katrin Ström
Deposited On:21 Apr 2005 00:00
Metadata Last Modified:02 Dec 2014 10:07

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