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Transcriptional responses during the pathogenic interaction between Heterobasidion Annosum sensu lato and conifers

Karlsson, Magnus (2005). Transcriptional responses during the pathogenic interaction between Heterobasidion Annosum sensu lato and conifers. Diss. (sammanfattning/summary) Uppsala : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae, 1652-6880 ; 2005:32
ISBN 91-576-7031-5
[Doctoral thesis]

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Abstract

This thesis summarizes the results of four studies regarding transcriptional changes of Heterobasidion annosum (Fr.) Bref. sensu lato and conifers in response to their pathogenic interaction. The diversity of expressed genes in H. annosum during the pathogenic interaction with Scots pine seedlings were studied by construction of a cDNA-library and generation of expressed sequence tags. Contig analysis identified 318 unigenes, of which 62 were repeatedly sampled. A putative function was assigned to 223 unigenes (70 %) that showed high or moderate similarity to protein sequences in public databases. The unigenes encoded proteins of metabolic functions with putative involvement in the pathogenic process, such as secondary metabolism, degradation of host material, signal perception and transduction and membrane transport. The transcriptional responses of several genes were studied with realtime-PCR during fungal infection of conifer material. Genes with a putative involvement in secondary metabolism, protection against oxidative stress and degradation/detoxification of host material were shown to be differentially expressed. A cytochrome P450 gene displayed sequence similarities towards genes encoding proteins involved in secondary metabolism and was highly expressed during fungal growth in Norway spruce bark. Up-regulation of a superoxide dismutase gene during early infection of Scots pine seedlings was detected. Transcript profiles of a laccase and two glutathione S-transferase genes showed transient expression during a time-coarse experiment of fungal infection of Norway spruce tissue cultures. A putative arabinase gene was exclusively expressed during infection of Scots pine seedlings. Up-regulation of Norway spruce genes phenylalanine ammonia lyase, peroxidase, glutathione S-transferase and class II and IV chitinases were detected upon fungal colonization of spruce callus tissue.

Authors/Creators:Karlsson, Magnus
Title:Transcriptional responses during the pathogenic interaction between Heterobasidion Annosum sensu lato and conifers
Year of publishing :2005
Volume:2005:32
Number of Pages:34
Papers/manuscripts:
NumberReferences
ALLI. Karlsson, M., Olson, Å. & Stenlid, J. 2003. Expressed sequences from the basidiomycetous tree pathogen Heterobasidion annosum during early infection of Scots pine. Fungal genetics and biology, 39, 51-59. II. Karlsson, M., Stenlid, J. & Olson, Å. Identification of a superoxide dismutase gene from the conifer pathogen Heterobasidion annosum and its infection-related regulation. (Submitted manuscript). III. Karlsson, M., Elfstrand, M., Stenlid, J. & Olson, Å. Differential gene expression during the interaction between Heterobasidion annosum sensu lato and its conifer hosts. (Manuscript). IV. Karlsson, M., Hietala, A., Kvaalen, H., Olson, Å., Solheim, H., Stenlid, J. & Fossdal, C. Infection-related transcriptional responses from the interaction of Norway spruce callus tissue and the fungal pathogen Heterobasidion parviporum. (Submitted manuscript).
Place of Publication:Uppsala
ISBN for printed version:91-576-7031-5
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Agris subject categories.:F Plant production > F30 Plant genetics and breeding
Subjects:Not in use, please see Agris categories
Agrovoc terms:heterobasidion annosum, pinus sylvestris, picea abies, gene expression, transcription, nucleotide sequence, cytochrome p450, oxidoreductases, glycosidases
Keywords:Heterobasidion annosum, Heterobasidion parviporum, Pinus sylvestris, Picea abies, expressed sequence tags, gene expression, cytochrome P450, glutathione S-transferase, arabinase.
URN:NBN:urn:nbn:se:slu:epsilon-616
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-616
ID Code:806
Department:(S) > Institutionen för skoglig mykologi och patologi
Deposited By: Magnus Karlsson
Deposited On:20 Apr 2005 00:00
Metadata Last Modified:02 Dec 2014 10:07

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