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Changes in the transcriptome and metabolome during the initiation of growth cessation in hybrid aspens

Hoffman, Daniel E. (2011). Changes in the transcriptome and metabolome during the initiation of growth cessation in hybrid aspens. Diss. (sammanfattning/summary) Umeå : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae, 1652-6880 ; 2011:43
ISBN 978-91-576-7587-3
[Doctoral thesis]

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Abstract

At 9 550 years of age, Old Rasmus, a Norway spruce (Picea abies), located in the Dalarna province in Sweden is recognized as the oldest surviving tree in the world. Old Rasmus exemplifies a robust capacity to survive in changing conditions, making trees a unique and fascinating subject for study. For perennial trees located in high latitudes one of the most important survival mechanisms is to be able to predict the coming winter and to halt growth in time. Poplars (Populus sp.) use mainly two environmental signals, temperature and light (day length), to predict the coming winter. However, of these two signals day length predominates. Towards the end of summer when the days shorten the trees recognize a critical day length and thereafter start the processes to halt growth and prepare for the impending winter.

This thesis focuses on the early gene expression and metabolic responses exhibited in hybrid aspen (Populus tremula L. x tremuloides) induced by a shorter day length below the critical day length. In our effort to identify and describe these responses we used high throughput technologies such as microarray and GC-MS to generate data. We show that similar responses exists in hybrid aspen as in Arabidopsis, with initial responses in carbohydrate and secondary metabolism pathways and circadian clock associated genes. Our results provide evidence that the hormone Gibberellin (GA) is linked with the photoperiodic regulation pathway by affecting PHYTOCHROME A (PHYA) expression and thereby the levels of transcripts that act downstream of PHYA. We also suggest that in Populus the protein GIGANTEA1 (GI1) might be able to influencing growth cessation process by regulating the expression of FLOWERING LOCUS T2 (FT2) independently of CONSTANS2 (CO2).

Authors/Creators:Hoffman, Daniel E.
Title:Changes in the transcriptome and metabolome during the initiation of growth cessation in hybrid aspens
Series/Journal:Acta Universitatis agriculturae Sueciae (1652-6880)
Year of publishing :2011
Volume:2011:43
Number of Pages:75
Papers/manuscripts:
NumberReferences
IHoffman, D. E., Jonsson, P., Bylesjo, M., Trygg, J., Antti, H., Eriksson, M. E., Moritz, T. (2010) Changes in diurnal patterns within the Populus transcriptome and metabolome in response to photoperiod variation. Plant, Cell & Environment 33: 1298-1313.
IIBylesjö, M.†, Eriksson, D.†, Kusano, M., Moritz, T., Trygg, J. (2007) Data integration in plant biology: the O2PLS method for combined modeling of transcript and metabolite data. Plant Journal 52: 1181-91
IIIBylesjö, M., Eriksson, D., Sjödin, A., Jansson, S., Moritz, T., Trygg, J. (2007) Orthogonal projections to latent structures as a strategy for microarray data normalization. BMC Bioinformatics 8:207.
IVHoffman, D. E.†, Eriksson, M. E.†, Kaduk, M., Mauriat, M., Moritz, T. (2011) GA biosynthesis is intimately linked with light reception and response pathways in photoperiodic control of growth in hybrid aspen. Manuscript.
VHoffman, D.E., Löfstedt, T., Böhlenius, H., Nilsson, O., Eriksson, M. E., Trygg, J., Moritz, T. (2011) Identifying early differences in the transcriptome and metabolome in RNAi-lines of PHYA and GI in response to short day induced growth cessation. Manuscript.
Place of Publication:Umeå
Publisher:Institutionen för skoglig genetik och växtfysiologi, Sveriges lantbruksuniversitet
ISBN for printed version:978-91-576-7587-3
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Biochemistry and Molecular Biology
Agrovoc terms:populus, hybrids, photoperiodicity, daylight, gibberellins, growth
Keywords:Populus, photoperiod, Gibberellin, PHYA, GI, FT
URN:NBN:urn:nbn:se:slu:epsilon-e-54
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-54
ID Code:8070
Department:(S) > Dept. of Forest Genetics and Plant Physiology
External funders:FORMAS and Knut and Alice Wallenberg Foundation and ["funders_typename_SLU.id.fund-slu" not defined] and Swedish Agency for Innovation Systems and Swedish Research Council
Deposited By: Daniel Hoffman
Deposited On:20 Apr 2011 08:51
Metadata Last Modified:02 Dec 2014 10:45

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