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Effect of wilting, silage additive, PEG treatment and tannin content on the distribution of N between different fractions after ensiling of three different sainfoin (Onobrychis viciifolia) varieties

Lorenz, Martin and Udén, Peter and Eriksson, Torsten (2010). Effect of wilting, silage additive, PEG treatment and tannin content on the distribution of N between different fractions after ensiling of three different sainfoin (Onobrychis viciifolia) varieties. Grass and forage science. 65:2, 175-184
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Official URL: http://dx.doi.org/10.1111/j.1365-2494.2010.00736.x

Abstract

Sainfoin (Onobrychis viciifolia) is a tanniniferous, leguminous plant that has potentially beneficial effects on protein utilization in ruminants. Since ensiling causes protein breakdown and elevated levels of buffer soluble N (BSN), we studied the distribution of N before and after ensiling sainfoin. Three varieties of sainfoin were either direct-cut and frozen directly or wilted and frozen before later ensiling in mini-silos with and without acidification with Promyr (PM; an acidifying commercial mixture of propionic and formic acid) and with or without polyethylene glycol (PEG). Extractable tannins (ET) and protein bound tannins (PBT) were measured with an HCl/butanol method in an attempt to correlate tannin levels to N fractions. The sainfoin silages showed good ensiling characteristics and had relatively high concentrations of un-degraded protein. The effect of wilting on BSN levels (g/kg N) was dependent on sainfoin variety (P<0.001). PEG increased and PM decreased the level of BSN in the silages (P<0.001). PM treatment also produced less non-protein N and ammonia-N (P<0.05) as compared with no additive. Addition of PEG to the silage increased the BSN-proportion 1.8- and 2.6-fold for both DM stages. A strong tannin-protein binding effect is, therefore, confirmed in sainfoin. However, correlations between tannin levels (ET and PBT) and BSN were poor in the (non-PEG) silages, indicating either that the HCl/butanol method is unsuitable for measuring tannin in silages or that qualitative attributes of tannins are more relevant than quantitative. The HCl/butanol method seems therefore not to be useful to predict degradation of protein in sainfoin silages.

Authors/Creators:Lorenz, Martin and Udén, Peter and Eriksson, Torsten
Title:Effect of wilting, silage additive, PEG treatment and tannin content on the distribution of N between different fractions after ensiling of three different sainfoin (Onobrychis viciifolia) varieties
Series/Journal:Grass and forage science (0142-5242)
Year of publishing :2010
Volume:65
Number:2
Page range:175-184
Publisher:Blackwell Publishing Ltd.
ISSN:0142-5242
Language:English
Publication Type:Journal article
Refereed:No
Article category:Scientific peer reviewed
Version:Submitted version
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 402 Animal and Dairy Science > Animal and Dairy Science.
Keywords:legume, sustainability, tannin
URN:NBN:urn:nbn:se:slu:epsilon-e-189
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-189
Additional ID:
Type of IDID
DOI10.1111/j.1365-2494.2010.00736.x
ID Code:8198
Department:(VH) > Dept. of Animal Nutrition and Management
External funders:EU FP6
Deposited By: Martin Lorenz
Deposited On:26 Aug 2011 14:29
Metadata Last Modified:02 Dec 2014 10:46

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