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Localization of carbonic anhydrase in reproductive organs

Ekstedt, Elisabeth (2005). Localization of carbonic anhydrase in reproductive organs. Diss. (sammanfattning/summary) Uppsala : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae, 1652-6880 ; 2005:40
ISBN 91-576-7039-0
[Doctoral thesis]

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Abstract

This thesis is a comparative study on localization of carbonic anhydrase (CA) in male and female reproductive organs. CA functions in pH and bicarbonate homeostasis and catalyzes the reaction CO₂ + H₂O ↔ HCO₃⁻ + H⁺. At present 15 different isozymes (CA I-XIV) have been characterized, three of them without catalytic activity. Only active isozymes are of physiological interest and a method demonstrating CA activity in tissues was used. Furthermore, a study on knockout mice deficient in CA II has been undertaken since these are used as model animals for the inherited human CA II deficiency syndrome. Tissues from testis and epididymis were taken from sexually mature pig, rabbit and mouse males; and from the ovary, oviduct and uterus from females of the same species and also cat. Corresponding tissues were taken from CA II-deficient mice. Tissues from other species were included when available from other studies. The tissues were fixed and processed for histochemical demonstration of CA activity and on mouse tissue also for immunohistochemical localization for CA II and CA IV. Spermatozoa were collected from testis, caput, corpus and cauda epididymidis and labelled for CA IV for scanning electron microscopy. Testis: The seminiferous epithelium was unstained except for the Sertoli cells in pig and acrosomes in hamster and lemming. The Leydig cells were CA positive in rat and pig. The capillaries were stained for CA activity in the testis of all species. Epididymis: The apical cells in caput showed CA activity in all species. The principal cells had basolateral membrane staining in cauda. The stereocilia/microvilli were stained in some regions in the species but not in pig. The membrane-bound CA IV was found to be transferred to the spermatozoa during passage through the duct. The capillaries were unstained. Ovary: The oocyte was unstained except for the rabbit cell membrane. The granulosa cells were stained only in the mouse and the theca interna was negative in all species. The luteal cells of corpus luteum were always unstained. The capillaries were variably stained for CA activity and unstained in mouse. Uterus: The surface epithelium was stained during at least some stage of the oestrus cycle in all species except pig. CA disappeared from the epithelium during the non-luteal phase in rabbit, while the glands were negative during the entire cycle. In the other species the glands were stained for CA activity. Stained capillaries were present in all species, except during the luteal phase in rabbit. CA II-deficient mouse: Membrane-bound CAs were more predominant than CA II in males, which may explain why the CA II-deficiency does not affect male fertility in the mouse. In the control females CA II was present in the uterine epithelium and glands as well as membrane-bound CA. The deficient females, however, often produce stillborn pups, which might be related to the lack of CA II in the uterine mucosa. Conclusions: The CA present in the epididymis is thought to be involved in the gradual acidification and bicarbonate resorption taking place in the duct. The envi4 ronment in cauda must have a low pH to keep the spermatozoa immotile during storage. The CA in the female is thought to have a function in the micro milieu in the oviduct and uterus during transport of spermatozoa and ovum and later for embryonic implantation in the uterus. However, the cyclic variation affects CA activity, which together with a significant species variation warrant further studies.

Authors/Creators:Ekstedt, Elisabeth
Title:Localization of carbonic anhydrase in reproductive organs
Year of publishing :April 2005
Volume:2005:40
Number of Pages:45
Papers/manuscripts:
NumberReferences
ALLI. Ekstedt, E., Ridderstråle, Y., Plöen, L. & Rodriguez-Martinez, H. 1991 Histochemical localization of carbonic anhydrase in the testis and epididymis of the boar. Acta Anatomica 141, 257-261. II. Ekstedt, E. & Ridderstråle, Y. 1992 Histochemical localization of carbonic anhydrase in the testis and epididymidis of the rabbit. Acta Anatomica 143, 258- 264. III. Rodriquez-Martinez, H., Ekstedt, E. & Ridderstråle, Y. 1991 Histochemical localization of carbonic anhydrase in the female genitalia of pigs during the oestrous cycle. Acta Anatomica 140, 41-47. IV. Ekstedt, E., Holm, L. & Ridderstråle, Y. 2004 Carbonic anhydrase in mouse testis and epididymis; transfer of isozyme IV to spermatozoa during passage. Journal of Molecular Histology 35, 16-173.
Place of Publication:Uppsala
ISBN for printed version:91-576-7039-0
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Agris subject categories.:L Animal production > L53 Animal physiology - Reproduction
Subjects:Not in use, please see Agris categories
Agrovoc terms:carbonate dehydratase, testes, ovaries, oviducts, uterus, spermatozoa
Keywords:Carbonic anhydrase, Histochemistry, Immunohistochemistry, Testis, Epididymis, Ovary, Oviduct, Uterus, CA II-deficient mice
URN:NBN:urn:nbn:se:slu:epsilon-625
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-625
ID Code:821
Department:(VH) > Dept. of Anatomy, Physiology and Biochemistry
Deposited By: Elisabeth Ekstedt
Deposited On:22 Apr 2005 00:00
Metadata Last Modified:02 Dec 2014 10:07

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