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Structural studies of some bacterial lipopolysaccharides and extracellular polysaccharides using NMR spectroscopy and mass spectrometry

Dag, Semiha (2005). Structural studies of some bacterial lipopolysaccharides and extracellular polysaccharides using NMR spectroscopy and mass spectrometry. Diss. (sammanfattning/summary) Uppsala : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae, 1652-6880 ; 2005:45
ISBN 91-576-7044-7
[Doctoral thesis]

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Abstract

The thesis presents structural studies of the O-specific chains from lipopolysaccharides, LPS, isolated from the bacteria Hafnia alvei strain PCM 1200, PCM 1203 and PCM 1205 and Plesiomonas shigelloides strain CNCTC 144/92 (O74:H5) as well as studies of extracellular polysaccharides, EPS, isolated from Butyrivibrio fibrisolvens strains I1 and 12. The main methods used, alone or in combination, for solving the structures have been chemical degradation procedures, mass spectrometry (MS) and nuclear magnetic resonance (NMR) spectroscopy. In the studies of Hafnia alvei it was established that the O-deacetylated O-specific polysaccharide isolated from LPS from H. alvei strains 1200 and 1203 possess the same composition and sequence as the O-deacetylated H. alvei strain 1205, i.e. a glycerol teichoic acid-like polymer having a repeating unit composed of a pentasaccharide unit. Serological tests revealed immunochemical similarities between the LPS of the Hafnia alvei strains PCM 1200, 1203 and 1205. In the studies of Plesiomonas shigelloides strain CNCTC 144/92 it was concluded that the O-specific chain is composed of a trisaccharide repeating unit with the following structure: [->2)-b-D-Quip3NHR-(1->3)-a-L-Rhap2OAc-(1->3)-a-D-FucpNAc-(1->] in which the R was determined as 3-hydroxy-2,3-dimethyl-5-oxo-pyrrolidine-2-carboxylic acid. The structure of the core part of the LPS was determined to be composed of a non-phosphorylated octasaccharide. The structure elucidation of the EPS produced by Butyrivibrio fibrisolvens strain I1 revealed a hexasaccharide repeating unit with a unique substituent consisting of glucitol phosphate where the glucitol was 4,6 substituted to a pyruvic acid acetal. The structure elucidation of the EPS produced by Butyrivibrio fibrisolvens strain 12 also revealed a hexasaccharide repeating unit with a unique substituent of glyceric acid phosphate where the glyceric acid was substituted with a glutamic acid.

Authors/Creators:Dag, Semiha
Title:Structural studies of some bacterial lipopolysaccharides and extracellular polysaccharides using NMR spectroscopy and mass spectrometry
Year of publishing :2005
Volume:2005:45
Number of Pages:49
Papers/manuscripts:
NumberReferences
ALLI. Dag S., Niedziela T., Dzieciatkowska M., Lukasiewicz J., Jachymek W., Lugowski C. and Kenne L. The O-acetylation patterns in the O-antigens of Hafnia alvei strains PCM 1200 and 1203, serologically closely related to PCM 1205. Carbohydr. Res. 2004, 339, 2521-2527 Reprint was made with permission from the publisher Elsevier II. Niedziela T., Dag S., Lukasiewicz J., Dzieciatkowska M., Jachymek W., Lugowski C. and Kenne L. Lipopolysaccharide of Plesiomonas shigelloides serogroup O74:H5 (Strain CNCTC 144/92). Structural analysis of the lipopolysaccharide including the O-antigen, its biological repeating unit, the core oligosaccharide, and the linkage between them. (Manuscript) III. Dag S., Cotta M. A. and Kenne L. Structural studies on the extracellular polysaccharide produced by Butyrivibrio fibrisolvens strain I1. (Manuscript) IV. Dag S., Cotta M. A. and Kenne L. Structural studies on the extracellular polysaccharide produced by Butyrivibrio fibrisolvens strain 12. (Manuscript)
Place of Publication:Uppsala
ISBN for printed version:91-576-7044-7
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Agris subject categories.:X Agricola extesions > X30 Life sciences
Subjects:Not in use, please see Agris categories
Agrovoc terms:hafnia, plesiomonas shigelloides, butyrivibrio, lipopolysaccharides, chemical structure, mass spectrometry, nmr spectroscopy
Keywords:Keywords: lipopolysaccharides, extracellular polysaccharides, NMR, MS, structural analysis, Hafnia alvei, Plesiomonas shigelloides, Butyrivibrio fibrisolvens.
URN:NBN:urn:nbn:se:slu:epsilon-626
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-626
ID Code:826
Department:(NL, NJ) > Dept. of Chemistry (until 131231)
Deposited By: Semiha Dag
Deposited On:26 Apr 2005 00:00
Metadata Last Modified:02 Dec 2014 10:07

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