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Genetic analysis of autoimmune and metabolic traits in chickens

Ek, Weronica (2011). Genetic analysis of autoimmune and metabolic traits in chickens. Diss. (sammanfattning/summary) Uppsala : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae, 1652-6880 ; 2011:73
ISBN 978-91-576-7617-7
[Doctoral thesis]

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Abstract

Studying how changes in DNA affect organisms and gene functions, can give us an increased understanding of the genetic regulation of diseases and metabolic traits. Chickens and other farm animals have long been used as a tool to study human diseases and other complex traits since they provide an excellent opportunity to study these traits in a controlled environment. In this thesis I have applied new statistical methods to experimental data from two outbred chicken crosses to investigate the genetic architecture and regulation of autoimmune and metabolic traits. The first cross was generated from two chicken lines divergently selected for high and low body weight and was used to study how a four-QTL network affects metabolic traits (papers I-III). The second cross was generated to study the genetic regulation of autoimmune systemic sclerosis (paper IV).

Our explorations of the effects of the four-QTL network on other metabolic traits indicate that the major contribution of the network is to increase body size through an overall increase in growth of all organs rather than by changing the relative size of different components. To be able to study the networks affect on metabolic traits further, we first generated and analysed an advanced intercross line to narrow down the regions. Once the regions were fine mapped, we designed and generated a so called introgression line. Three of the fine mapped QTL were transferred (introgressed) from the low weight line into a high weight line background. This introgression line can be used to further investigate the QTL network. In the second cross, three suggestive autosomal susceptibility QTL were mapped in the chicken genome, with several orthologues of human systemic sclerosis candidate genes located within two of these loci. We also observed less disease in females with a W chromosome inherited from the diseased founder line.

Authors/Creators:Ek, Weronica
Title:Genetic analysis of autoimmune and metabolic traits in chickens
Series/Journal:Acta Universitatis agriculturae Sueciae
Year of publishing :16 September 2011
Volume:2011:73
Number of Pages:70
Papers/manuscripts:
NumberReferences
I.Weronica Ek, Lina Strömstedt, Per Wahlberg, Paul Siegel, Leif Andersson & Örjan Carlborg (2010). Genetic analysis of metabolic traits in an intercross between body weight selected chicken lines. Physiol Genomics 42;20-22. doi:10.1152/physiolgenomics.00149.2009
II.Francois Besnier, Per Wahlberg, Lars Rönnegård, Weronica Ek, Leif Andersson, Paul B Siegel & Örjan Carlborg (2010). Fine mapping and replication of QTL in outbred Advance Intercross Line. Genetics Selection Evolution 2011;43:3. BioMed Central
III.Weronica Ek, Stefan Marklund, Ashok Ragawand, Paul Siegel, William Muir & Örjan Carlborg (2011). Generation of a multi-locus chicken introgression line to study the effects of genetic interactions on metabolic phenotypes in chicken. Manuscript.
IV.Weronica Ek, Anna-Stina Sahlqvist, Lucy Crooks, Roswitha Sgonc, Hermann Dietrich, George Wick, Olov Ekvall, Leif Andersson, Örjan Carlborg, Olle Kämpe & Susanne Kerje (2011). Mapping Scleroderma genes in an outbred chicken intercross. Submitted.
Place of Publication:Uppsala
Publisher:Dept. of Animal Breeding and Genetics, Swedish University of Agricultural Sciences
ISBN for printed version:978-91-576-7617-7
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Agris subject categories.:L Animal production > L10 Animal genetics and breeding
Subjects:Obsolete subject words > FORESTRY, AGRICULTURAL SCIENCES and LANDSCAPE PLANNING > Animal production > Animal breeding
Obsolete subject words > VETERINARY MEDICINE > Cell biology and genome research
Obsolete subject words > VETERINARY MEDICINE > Microbiology and immunology
Agrovoc terms:chickens, gene interaction, metabolism, immunity, disease control
Keywords:chicken, QTL, epistasis, metabolic, autoimmune
URN:NBN:urn:nbn:se:slu:epsilon-e-192
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-192
ID Code:8291
Department:(VH) > Dept. of Animal Breeding and Genetics
External funders:FORMAS
Deposited By: MsC Weronica Ek
Deposited On:30 Aug 2011 09:00
Metadata Last Modified:02 Dec 2014 10:47

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