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Borna disease virus and its hosts

studies on virus-host interactions and virus detection

Wensman, Jonas (2011). Borna disease virus and its hosts. Diss. (sammanfattning/summary) Uppsala, Sweden : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae, 1652-6880 ; 2011:62
ISBN 91-576-7606-1
[Doctoral thesis]

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Abstract

Borna disease virus (BDV) is an RNA-virus persistently infecting the central nervous system of, and thereby causing neurological disorders in, several animal
species, possibly including humans. Although several aspects of BDV and its pathologies have been studied for more than 100 years, further elucidation of the
epidemiology and pathogenesis, and the development of reliable detection methods,is needed.

This thesis describes the relationship between BDV and its hosts, with special emphasis on virus-host interactions and virus detection. The aims were to improve current detection methods, and to increase the knowledge of virus-host interactions, especially in naturally infected animals. First, a real-time RT-PCR assay for BDV detection was developed; and second this method, as well as serology, was evaluated for use when diagnosing Borna disease (BD) in cats. These studies suggest that serology is the most useful tool to further strengthen a clinical suspicion of feline BD, whereas molecular diagnostics, using blood samples, can be added for cats showing mild neurological signs.

In the third study, phosphorylated BDV P and previously reported BDV-host protein-protein interactions were detected in cell cultures, as well as in experimentally and naturally infected animals, by the use of an in situ proximity ligation assay. These protein-protein interactions most likely interfere with signaling
pathways of the host, enabling BDV to establish a persistent infection.

The type II interferon (IFN-!) is an important key factor of the antiviral host immune response, involved in non-cytolytic clearance of BDV. In the final study, IFN-! mRNA expression was studied in feline BD. A high expression was seen in
these cats, as compared to non-infected controls. This expression was higher in cats
showing mild neurological signs, and in these cats also moderate-severe pathological
lesions were found. These data point to more direct effects of BDV in disease development than has previously been considered.

In conclusion, this thesis provides an overview of the current knowledge of BDV, and contributes novel data on virus-host interactions and virus detection.

Authors/Creators:Wensman, Jonas
Title:Borna disease virus and its hosts
Subtitle:studies on virus-host interactions and virus detection
Series/Journal:Acta Universitatis agriculturae Sueciae (1652-6880)
Year of publishing :2011
Volume:2011:62
Number of Pages:89
Papers/manuscripts:
NumberReferences
IWensman, J.J., Thorén, P., Hakhverdyan, M., Belák, S., and Berg, M. (2007). Development of a real-time RT-PCR assay for improved detection of Borna disease virus. Journal of Virological Methods 143(1), 1-10.
IIWensman, J.J., Jäderlund, K.H., Gustavsson, M.H., Hansson-Hamlin, H., Karlstam, E., Lilliehöök, I., Öström, I-L.Ö., Belák, S., Berg, M., and Holst, B.S. (2011). Diagnostic markers in feline Borna disease. Submitted.
IIIWensman, J.J., Leuchowius, K-J., Yan, J., Berg, A-L., Ludwig, H., Bode, L., Belák, S., Landegren, U., Söderberg, O., and Berg, M. (2011). Visualization of Borna disease virus protein interactions with host proteins using in situ proximity ligation assay. (Manuscript).
IVWensman, J.J., Ilbäck, C., Hjertström, E., Blomström, A-L., Gustavsson, M.H., Jäderlund, K.H., Ström-Holst, B., Belák, S., Berg, A-L., and Berg, M. (2011). Expression of interferon gamma in brain tissues of cats with natural Borna disease virus infection. Veterinary Immunology and Immunopathology 141(1-2), 162-167.
Place of Publication:Uppsala, Sweden
Publisher:Institutionen för biomedicin och veterinär folkhälsovetenskap, Sveriges lantbruksuniversitet
ISBN for printed version:91-576-7606-1
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Agris subject categories.:L Animal production > L10 Animal genetics and breeding
L Animal production > L70 Veterinary science and hygiene - General aspects
L Animal production > L73 Animal diseases
Subjects:Obsolete subject words > VETERINARY MEDICINE > Microbiology and immunology
Agrovoc terms:borna disease virus, animal viruses, rna, disease control, diagnosis, disease transmission
Keywords:borna disease virus, staggering disease, persistent infection, protein-protein interactions, IFN-gamma, in situ proximity ligation assay, diagnostics
URN:NBN:urn:nbn:se:slu:epsilon-e-199
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-199
ID Code:8311
Department:(VH) > Dept. of Biomedical Sciences and Veterinary Public Health
Deposited By: Jonas Wensman
Deposited On:05 Sep 2011 12:37
Metadata Last Modified:02 Dec 2014 10:47

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