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Setosphaeria turcica, fungal mating and plant defence

Martin, Tom (2011). Setosphaeria turcica, fungal mating and plant defence. Diss. (sammanfattning/summary) Uppsala : Sveriges lantbruksuniv., Acta Universitatis Agriculturae Sueciae, 1652-6880 ; 2011:66
ISBN 978-91-576-7610-8
[Doctoral thesis]

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The heterothallic ascomycete Setosphaeria turcica (anamorph: Exserohlium turcicum) causes turcicum leaf blight on maize and sorghum. A survey was undertaken in Uganda to examine the sorghum – S. turcica interaction in terms of disease severity and incidence, overall fungal population structure, and new resistant resources. Highest disease severities were recorded on caudatum accessions, whereas kafir genotypes were most resistant. Highly resistant sorghum accessions originating from a regional collection were found among the five local sorghum races. The disease was more severe in the most humid farmlands. Upon cross inoculation on maize differential lines, S. turcica isolates corresponding to race 0, 1, 2 and 3 were found, increasing the number of known races in Uganda. The two S. turcica mating type genes MAT1-1 and MAT1-2 were found in 20 of 23 districts sampled and in equal proportions on sorghum and maize indicating that sexual recombination is present in Uganda. Fungal mating types in Pezizomycotina are characterised by genes encoding either an HMG or α1 domain protein, occupying the same locus on corresponding chromosomes. We present sequence comparisons, phylogenetic analyses, and in silico predictions of secondary and tertiary structures, which support our hypothesis that the α1 domain is related to the HMG domain and share a common ancestor. We have also characterized a new conserved motif in α1 proteins of Pezizomycotina. This motif is immediately adjacent to and downstream of the α1 domain. The S. turcica genome contains 123 unique protein sequences not found in related fungi. These are of importance for plant cell wall degradation, ion-binding and transport. Genome comparisons of maize versus Brassica infecting fungi revealed 628 maize specific protein groups including a number of potential effectors.

Six NB-LRR encoding St genes residing in three pairs in one locus on chromosome 5 in sorghum were found to mediate resistance to S. turcica. The St gene homologs have all highly conserved sequences, and commonly reside as gene pairs in the grass genomes.

Authors/Creators:Martin, Tom
Title:Setosphaeria turcica, fungal mating and plant defence
Series Name/Journal:Acta Universitatis Agriculturae Sueciae
Year of publishing :8 September 2011
Number of Pages:53
I.Ramathani, I., Biruma, M., Martin, T., Dixelius, C. and Okori, P. (2011) Disease severity, incidence and races of Setosphaeria turcica on sorghum in Uganda. Eur. J. Plant Pathol. DOI 10.1007/s10658-011-9815- 1.
II.§Martin, T., §Lu, S-W., van Tilbeurgh, H., Ripoll, DR., Dixelius, C., Turgeon, GB. and Debuchy, R. (2010) Tracing the origin of the fungal α1 domain places its ancestor in the HMG-box superfamily: implication for fungal mating-type evolution, PLoS ONE. DOI 10.1371/5:e15199.
III.Martin, T., Schwelm, A. and Dixelius, C. Genome-wide comparative analysis reveals insight into maize fungal pathogens. (Manuscript).
IV.Martin, T., Biruma, M., Fridborg, I., Okori, P. and Dixelius, C. (2011) A highly conserved NB-LRR encoding gene cluster effective against Setosphaeria turcica in sorghum. BMC Plant Biol. (Accepted pending revision).
Place of Publication:Uppsala
Publisher:Dept. of Plant Biology and Forest Genetics, Swedish University of Agricultural Sciences
ISBN for printed version:978-91-576-7610-8
Publication Type:Doctoral thesis
Full Text Status:Public
Agris subject categories.:H Protection of plants and stored products > H20 Plant diseases
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 404 Agricultural Biotechnology > Plant Biotechnology
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 404 Agricultural Biotechnology > Genetics and Breeding
Agrovoc terms:setosphaeria turcica, blights, fungal diseases, evolution, genes, zea mays, sorghum, uganda
Keywords:Exserohlium turcicum, Maize, Mating type, R-genes, Sorghum, turcicum leaf blight
Permanent URL:
ID Code:8317
Department:(NL, NJ) > Dept. of Plant Biology and Forest Genetics (until 131231)
Deposited By: Mr Tom Martin
Deposited On:09 Sep 2011 11:38
Metadata Last Modified:02 Dec 2014 10:47

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