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Molecular regulation of embryo development in Norway spruce

polar auxin transport and transcription factors

Larsson, Emma (2011). Molecular regulation of embryo development in Norway spruce. Diss. (sammanfattning/summary) Uppsala : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae, 1652-6880 ; 2011:68
ISBN 978-91-576-7612-2
[Doctoral thesis]

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Abstract

Early events in embryo development are critical for the plant body formation. During this phase the apical-basal axis, the radial symmetry, and the primary meristems are specified. The shoot apical meristem (SAM) and the root apical meristem (RAM) will subsequently give rise to all above-ground and below-ground tissues. Despite the environmental and economical importance of conifers, the regulation of their embryonic development is still relatively unknown. We are using somatic embryos of Norway spruce as a model system for studying embryology in conifers.

In this thesis, I show that polar auxin transport (PAT) is essential for proper patterning of both apical and basal parts of conifer embryos. Blocked PAT caused increased auxin levels, increased differentiation of early somatic embryos, a skewed balance between the embryonal mass and the suspensor cells in late embryos, and an abnormal morphology with irregular RAM, fused or aborted cotyledons and absence of a functional SAM in mature embryos.

Two NAC gene family members (PaNAC01 and PaNAC02), closely related to the Arabidopsis CUP-SHAPED COTYLEDON (CUC) genes, were characterized. PaNAC01 harbors previously characterized functional motifs, and could complement the cuc1cuc2 double mutant. The temporal expression of PaNAC01 was dependent on PAT, and coincided with the formation of separated cotyledons and a functional SAM. Furthermore, the expression profiles of four KNOXI genes (HBK1 to 4) showed that also the expression of HBK2 and HBK4 depend on PAT, and indicated their role in embryo differentiation and SAM formation, while HBK1 and HBK3 seemingly have a more general role during embryo development.

In addition, a global gene expression analysis revealed important processes during early somatic embryogenesis in Norway spruce.

Taken together the results show that central parts of the regulatory network for embryo development are still conserved between angiosperms and gymnosperms, despite their separation 300 million years ago.

Authors/Creators:Larsson, Emma
Title:Molecular regulation of embryo development in Norway spruce
Subtitle:polar auxin transport and transcription factors
Series/Journal:Acta Universitatis agriculturae Sueciae (1652-6880)
Year of publishing :September 2011
Volume:2011:68
Number of Pages:63
Papers/manuscripts:
NumberReferences
I.Larsson, E., Sitbon, F., Ljung, K., von Arnold, S. (2008). Inhibited polar auxin transport results in aberrant embryo development in Norway spruce. New Phytologist 177, 356-366.
II.Larsson, E., Sundström, JF., Sitbon, F., von Arnold, S. Characterisation of two CUP-SHAPED COTYLEDON orthologues (PaNAC01 and PaNAC02) in Picea abies (Norway spruce). Manuscript
III.Larsson, E., Sitbon, F., von Arnold, S. Expression of Knotted-1 like genes during the establishment of the shoot apical meristem in Picea abies (Norway spruce). Manuscript
IV.Vestman, D., Larsson, E., Uddenberg, D., Cairney, J., Clapham, D., Sundberg, E., von Arnold, S. (2011). Important processes during differentiation and early development of somatic embryos of Norway spruce as revealed by changes in global gene expression. Tree Genetics and Genomes 7, 347–362.
AppendixLarsson, E., Sitbon, F., von Arnold, S. (2008). Polar auxin transport controls suspensor fate. Plant Signaling and Behavior 3:7, 469-470.
Place of Publication:Uppsala
Publisher:Dept. of Plant Biology and Forest Genetics, Swedish University of Agricultural Sciences
ISBN for printed version:978-91-576-7612-2
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Agris subject categories.:F Plant production > F62 Plant physiology - Growth and development
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Forest Science
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 404 Agricultural Biotechnology > Plant Biotechnology
Agrovoc terms:picea abies, plant embryos, somatic embryogenesis, embryonic development, auxins, gene transcription, transcription factors, apical meristems
Keywords:auxin, cotyledons, CUC, conifers, KNOX, NAC, Norway spruce, polar auxin transport (PAT), shoot apical meristem (SAM), somatic embryogenesis
URN:NBN:urn:nbn:se:slu:epsilon-e-206
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-206
ID Code:8321
Department:(NL, NJ) > Dept. of Plant Biology and Forest Genetics (until 131231)
External funders:FORMAS
Deposited By: Emma Larsson
Deposited On:09 Sep 2011 12:08
Metadata Last Modified:02 Dec 2014 10:47

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