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Alkylresorcinol metabolites

candidate biomarkers for whole grain wheat and rye intake

Marklund, Matti (2012). Alkylresorcinol metabolites. Diss. (sammanfattning/summary) Uppsala : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae, 1652-6880 ; 2012:24
ISBN 978-91-576-7660-3
[Doctoral thesis]

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Abstract

Alkylresorcinols (AR), a group of phenolic lipids present in the outer parts of wheat and rye, have been evaluated as biomarkers for the intake of whole grain (WG) products of these cereals. AR may be utilized to facilitate the investigation of diet-disease associations in epidemiological studies. In the body, AR are metabolized to two phenolic acids, DHBA and DHPPA, which can be detected in plasma and urine, either as such or as more polar conjugates. The aim of this thesis was to evaluate AR metabolites DHBA and DHPPA as biomarkers for the intake of WG wheat and rye.

A gas chromatographic – mass spectrometric method was established that allowed the reliable quantification of AR metabolites in urine samples. This method was comparable to a previously established HPLC-CEAD method. Plasma pharmacokinetics of AR and their metabolites were compared in rats administered intact AR orally or AR metabolites intravenously. The elimination was faster for AR metabolites than for intact AR and the elimination half-lives increased linearly with the number of carbon atoms in the side chain. The formation of metabolites did not differ between rats given shorter or longer AR homologues, indicating that the different homologues were absorbed to a similar extent. Several anthropometric and dietary factors were identified by multivariate statistical tools as potential determinants of urinary AR metabolites and were hypothesized to influence the formation and excretion of AR metabolites at different stages during AR elimination. The reproducibility and relative validity of urinary AR metabolites as biomarkers for the intake of whole grain wheat and rye were assessed in Swedish men and women consuming their habitual diet. AR metabolites were well correlated to self-reported intakes of WG and cereal fibre. The reproducibility of urinary AR metabolites over 2-3 months was dependent on the stability of intake and was higher in 24-h urine collections compared to spot samples.

Due to the scarcity of 24-h urine collections in large observational studies and the modest reproducibility in plasma and spot urine samples, the utility of single AR metabolite measurements alone to reflect long-term intakes in epidemiological studies is limited. However, other applications of AR metabolites as biomarkers might prove useful in future studies (e.g., as a compliance check in intervention studies or as reference methods in validations of other dietary assessment tools).

Authors/Creators:Marklund, Matti
Title:Alkylresorcinol metabolites
Subtitle:candidate biomarkers for whole grain wheat and rye intake
Series/Journal:Acta Universitatis agriculturae Sueciae (1652-6880)
Year of publishing :30 March 2012
Volume:2012:24
Number of Pages:78
Papers/manuscripts:
NumberReferences
I.Marklund M, Landberg R, Åman P, Kamal-Eldin A. (2010). Determination of alkylresorcinol metabolites in human urine by gas chromatography-mass spectrometry. Journal of Chromatography B 878(11-12), 888-894.
II.Marklund M, Landberg R, Åman P, Kamal-Eldin A. (2011). Comparison of gas chromatography-mass spectrometry and high-performance liquid chromatography with coulometric electrode array detection for determination of alkylresorcinol metabolites in human urine. Journal of Chromatography B 879(9-10), 647-651.
III.Marklund M, Strömberg E, Hooker A, Hammarlund-Udenaes M, Åman P, Landberg R, Kamal-Eldin A. Plasma pharmacokinetics of alkylresorcinols and their metabolites in rats (manuscript).
IV.Marklund M, Landberg R, Andersson R, Åman P, Kamal-Eldin A. Alkylresorcinol metabolism is affected by factors other than intake of whole grain wheat and rye (submitted).
V.Marklund M, Landberg R, Andersson A, Åman P, Kamal-Eldin A. (2012). Alkylresorcinol metabolites in urine correlate with intake of whole grains and cereal fibre in free-living Swedish adults. British Journal of Nutrition (accepted for publication).
Place of Publication:Uppsala
Publisher:Dept. of Food Science, Swedish University of Agricultural Sciences
ISBN for printed version:978-91-576-7660-3
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Agris subject categories.:Q Food science > Q04 Food composition
S Human nutrition > S01 Human nutrition - General aspects
X Agricola extesions > X50 Chemistry
Subjects:Obsolete subject words > NATURAL SCIENCES > Chemistry > Analytical chemistry
Obsolete subject words > FORESTRY, AGRICULTURAL SCIENCES and LANDSCAPE PLANNING > Product science > Food science
(A) Swedish standard research categories 2011 > 3 Medical and Health Sciences > 303 Health Sciences > Nutrition and Dietetics
Obsolete subject words > NATURAL SCIENCES > Chemistry > Analytical chemistry > Separation methods
Agrovoc terms:alkylresorcinols, metabolites, phenolic acids, wheats, rye, food intake, digestive absorption, analytical methods
Keywords:Alkylresorcinol, biomarker, DHBA, DHPPA, metabolites, rye, wheat, whole grain
URN:NBN:urn:nbn:se:slu:epsilon-e-389
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-389
ID Code:8680
Department:(NL, NJ) > Dept. of Food Science (until 161231)
External funders:FORMAS
Deposited By: Matti Marklund
Deposited On:02 Apr 2012 09:24
Metadata Last Modified:02 Dec 2014 10:49

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