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Genetic variation, biochemical contents and wound healing activity of Plantago major

Zubair, Muhammad (2012). Genetic variation, biochemical contents and wound healing activity of Plantago major. Diss. (sammanfattning/summary) Alnarp, Sweden : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae, 1652-6880 ; 2012:20
ISBN 978-91-576-7656-6
[Doctoral thesis]

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Abstract

Plantago major L. (greater plantain, common plantain) has been used as a wound healing remedy in different parts of the world for centuries. Different bioactive compounds have been proposed to contribute to the wound healing properties of this plant. The present study was undertaken to investigate the impact of some genetic and environmental factors on the wound healing activity of common plantain.
Seeds of P. major were collected from five populations in different parts of Sweden, and were germinated and grown in a greenhouse. As expected for an inbreeding species, RAPD analyses demonstrated considerable between-population variation but very sparse within-population and within-subpopulation variation. Six major phenolic compounds were encountered in samples of P. major, four of which were identified for the first time in this thesis; PLMA 1–PLMA 4. Between-population and sub-population differences in the contents of these chemical compounds showed no correlation with RAPD-based estimates of genetic relatedness. The contents of these compounds differed greatly between different plant organs of P. major. The highest concentration of plantamajoside and PLMA 2 was found in leaves whereas the highest concentration of verbascoside was found in flower stalks and seeds. Contents were significantly higher in freeze-dried leaf samples compared to samples dried at higher temperatures. Both water and ethanol-based extracts of P. major leaves stimulated the cell proliferation and migration in an in vitro scratch assay, and also showed anti-inflammatory activity in an in vitro NF-kB assay with oral epithelial cell cultures. Similarly, these extracts stimulated wound healing activities in ex vivo tests using detached pig ears. Further breeding efforts aimed at developing P. major as a crop plant, and medicinal research aimed at elucidating and optimizing extracts with wound healing properties, are thus warranted.

Authors/Creators:Zubair, Muhammad
Title:Genetic variation, biochemical contents and wound healing activity of Plantago major
Series/Journal:Acta Universitatis agriculturae Sueciae (1652-6880)
Year of publishing :2 April 2012
Volume:2012:20
Number of Pages:47
Papers/manuscripts:
NumberReferences
IZubair, M., Nybom, H., Lindholm, C. & Rumpunen, K. 2011. Major polyphenols in aerial organs of greater plantain (Plantago major L.), and effects of drying temperature on polyphenol contents in the leaves. Scientia Horticulturae 128, 523–529.
IIZubair, M., Ekholm, A., Nybom, H., Renvert, S., Widén, C. & Rumpunen, K. (In press). Effects of Plantago major L. leaf extracts on oral epithelial cells in a scratch assay. Journal of Ethnopharmacology.
IIIZubair, M., Ekholm, A., Nybom, H., Renvert, S., Widén, C. & Rumpunen, K. (In press). Effects of Plantago major L. leaf extracts on oral epithelial cells in a scratch assay. Journal of Ethnopharmacology.
IVZubair, M., Nybom, H., Lindholm, C., Brandner, J.M. & Rumpunen, K. (manuscript submitted). Promotion of wound healing by Plantago major L. leaf extracts – ex vivo experiments confirm experiences from traditional medicine.
VZubair, M., Widén, C., Renvert, S., & Rumpunen, K. (manuscript). Antiinflammatory activity of Plantago major L. leaf extracts on oral epithelial cells.
Place of Publication:Alnarp, Sweden
Publisher:Dept. of Plant Breeding and Biotechnology, Swedish University of Agricultural Sciences
ISBN for printed version:978-91-576-7656-6
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Agris subject categories.:F Plant production > F60 Plant physiology and biochemistry
Subjects:(A) Swedish standard research categories 2011 > 3 Medical and Health Sciences > 304 Medical Biotechnology > Other Medical Biotechnology
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 404 Agricultural Biotechnology > Plant Biotechnology
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 404 Agricultural Biotechnology > Genetics and Breeding
Agrovoc terms:plantago, genetic variation, drug plants, healing, rapd, biochemical compounds
Keywords:polyphenol, plantain, RAPD , scratch assay, wound healing
URN:NBN:urn:nbn:se:slu:epsilon-e-395
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-395
ID Code:8685
Department:(LTJ, LTV) > Plant Breeding and Biotechnology (until 121231)
Deposited By: Mr. Muhammad Zubair
Deposited On:02 Apr 2012 14:45
Metadata Last Modified:02 Dec 2014 10:49

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