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Diversity and interactions of begomoviruses and their associated DNA-satellites

Sattar, Muhammad Naeem (2012). Diversity and interactions of begomoviruses and their associated DNA-satellites. Diss. (sammanfattning/summary) Uppsala : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae, 1652-6880 ; 2012:23
ISBN 978-91-576-7659-7
[Doctoral thesis]

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Abstract

To study the diversity of begomovirus/DNA-satellite complexes, plants of one crop species (okra; Abelmoschus esculentus) and one weed species (Ageratum conyzoides), both with leaf curling symptoms, were analyzed. Sequence analyses of complete genome components revealed a unique begomovirus/DNA-satellite complex in both plants. Okra was found to harbor infection with viruses of three begomovirus species, including the new recombinant species Okra leaf curl Cameroon virus, as well as one betasatellite and two divergent alphasatellites. The analysis of A. conyzoides revealed infection with a previously un-described complex of a begomovirus, betasatellite and alphasatellite. The results suggest that the diversity of begomoviruses and their associated DNA-satellites in Africa is much higher than previously thought, and that it may be similar to the diversity in Asia. Begomoviruses and satellites from different geographic regions, such as Africa and Asia, are genetically different. To test the compatibility of begomoviruses and satellites from Asia, Africa and the Mediterranean Region, inoculation experiments were carried out in the model host Nicotiana benthamiana using agroinfectious constructs for different begomoviruses and satellites. It was found that the tested begomoviruses and satellites could functionally interact with non-cognate betasatellites and alphasatellites with varying efficiencies. The results showed that the functional requirements for interaction between begomovirus and satellite can be quite relaxed. Thus, it is quite possible that new begomovirus-satellite combinations with components from different geographic regions will be formed, which may overcome plant resistance and increase the host range of begomoviruses. Functional studies were carried out on the betasatellite-associated monopartite begomovirus Cotton leaf curl Kokhran virus. Mutation analyses of the coat protein (CP), V2, C2 and C4 genes showed that CP, V2 and C2 are pathogenicity determinants and that they are involved in facilitating virus movement and maintenance of betasatellites. C4 is responsible for symptom induction, but it was not specifically required to maintain betasatellites.

Authors/Creators:Sattar, Muhammad Naeem
Title:Diversity and interactions of begomoviruses and their associated DNA-satellites
Series/Journal:Acta Universitatis agriculturae Sueciae (1652-6880)
Year of publishing :April 2012
Volume:2012:23
Number of Pages:74
Papers/manuscripts:
NumberReferences
I.Leke W.N., Brown J.K., Ligthart M.E., Sattar N., Njualem D.K., Kvarnheden A. (2012). Ageratum conyzoides: A host to a unique begomovirus disease complex in Cameroon. Virus Research 163, 229-237.
II.Leke W.N., Sattar M.N., Ngane E.B., Ngeve J.M., Kvarnheden A., Brown J.K. Diverse helper begomoviruses and satellite DNAs infecting okra plants in Cameroon. Manuscript
III.Sattar M.N., Ligthart M.E., Kvarnheden A. Molecular comparisons and trans-replication studies of begomoviruses and DNA satellites causing leaf curl disease in Asia and Africa. Manuscript
IV.Iqbal Z., Sattar M.N., Kvarnheden A., Mansoor M., Briddon R.W. Effects of the mutation of selected genes of Cotton leaf curl Kokhran virus on infectivity, symptoms and the maintenance of Cotton leaf curl Multan betasatellite. Manuscript
Place of Publication:Uppsala
Publisher:Department of Plant Biology and Forest Genetics, Swedish University of Agricultural Sciences
ISBN for printed version:978-91-576-7659-7
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Agris subject categories.:F Plant production > F30 Plant genetics and breeding
H Protection of plants and stored products > H20 Plant diseases
Subjects:Obsolete subject words > FORESTRY, AGRICULTURAL SCIENCES and LANDSCAPE PLANNING > Plant production > Plant and forest protection
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 404 Agricultural Biotechnology > Plant Biotechnology
Agrovoc terms:plant viruses, cotton leaf curl virus, ageratum conyzoides, abelmoschus esculentus, okras, nicotiana, host pathogen relations, genetic variation, genomes, dna, cameroon, asia
Keywords:Plant disease, mixed infection, begomovirus, DNA satellites, interaction, trans-replication, mutation, Nicotiana benthamiana
URN:NBN:urn:nbn:se:slu:epsilon-e-427
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-427
ID Code:8734
Department:(NL, NJ) > Dept. of Plant Biology and Forest Genetics (until 131231)
Deposited By: Mr. Naeem Sattar Muhammad
Deposited On:25 Apr 2012 07:18
Metadata Last Modified:02 Dec 2014 10:49

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