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Changes in global gene expression and auxin dynamics during embryo development in conifers

Vestman, Daniel (2012). Changes in global gene expression and auxin dynamics during embryo development in conifers. Uppsala : Sveriges lantbruksuniv.
ISBN 978-91-576-9089-0
[Licentiate thesis]

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Abstract

Conifers are of great economical importance, but our knowledge about how the development of conifers is regulated is limited. The over-all aim of this study has been to gain a better understanding of the molecular regulation of embryo development in conifers. This can in turn lead to improved methods in forest tree biotechnology and give insights into the evolution of land-plants. We have used somatic embryos to study early stages during embryo development, with a focus on global changes in gene expression and auxin dynamics.

Global changes in gene expression during early embryo development in Norway spruce (Picea abies) were analyzed by using a microarray spotted with 12,536 cDNA clones. This allowed us to pinpoint important molecular events like the establishment of different domains and boundary regions, "nurse cells" having a megagametophyte signaling function and a switch from embryonal to vegetative development.

Utilizing the same microarray production used for Norway spruce, we studied comparable stages during Scots pine (Pinus sylvestris) early embryo development, and identified processes such as the initiation of pattern formation. A comparison with the Norway spruce microarray revealed a possible delay in the onset of programmed cell death and polar auxin transport. These are differences that could account for problems with recalcitrance in Scots pine.

Two auxin responsive reporter constructs were used to monitor the dynamics of auxin during embryo development in Norway spruce. We could show that auxin is first synthesized and accumulated in the suspensor of early embryos, and that the site of synthesis is shifted to the embryonal mass in late embryos while polar auxin transport relocates the auxin in a basal direction. In maturing embryos, auxin is synthesized in the basal part and distributed throughout the embryo by polar transport.

Taken together, this study has revealed important processes during embryo development in Norway spruce and Scots pine and also shown how auxin is dynamically distributed during embryo development in Norway spruce.

Authors/Creators:Vestman, Daniel
Title:Changes in global gene expression and auxin dynamics during embryo development in conifers
Year of publishing :2012
Number of Pages:39
Papers/manuscripts:
NumberReferences
I.Vestman, D., Larsson, E., Uddenberg, D., Cairney, J., Clapham, D., Sundberg, E., von Arnold, S. (2010). Important processes during differentiation and early development of somatic embryos of Norway spruce as revealed by changes in global gene expression. Tree Genetics & Genomes 7(2), 347-362.
II.Vestman, D., Abrahamsson, M., Larsson, E., Cairney, J., Sundberg, E., Clapham, D., von Arnold, S. Global changes in gene expression during differentiation of early somatic embryos and development of late embryos in Scots pine. Manuscript
III.Vestman, D., Larsson, E., Sundberg, E., von Arnold, S. Local auxin activity is crucial during embryo development in conifers. Manuscript (preliminary)
Place of Publication:Uppsala
Publisher:Institutionen för växtbiologi och skogsgenetik, Sveriges lantbruksuniversitet
ISBN for printed version:978-91-576-9089-0
Language:English
Publication Type:Licentiate thesis
Full Text Status:Public
Agris subject categories.:F Plant production > F30 Plant genetics and breeding
F Plant production > F62 Plant physiology - Growth and development
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Cell Biology
(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Developmental Biology
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Forest Science
Agrovoc terms:picea abies, pinus sylvestris, embryonic development, auxins, gene expression, somatic embryogenesis
Keywords:Conifer, Early embryogeny, Late embryogeny, Microarray, Norway spruce, Scots pine, Somatic embryogenesis, Polar auxin transport, GH3
URN:NBN:urn:nbn:se:slu:epsilon-e-573
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-573
ID Code:8970
Department:(NL, NJ) > Dept. of Plant Biology and Forest Genetics (until 131231)
External funders:Swedish Research Council
Deposited By: Daniel Vestman
Deposited On:22 Aug 2012 08:35
Metadata Last Modified:02 Dec 2014 10:51

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