Home About Browse Search
Svenska


Molecular characterization and detection of infectious bronchitis virus

Abro, Shahid Hussain (2012). Molecular characterization and detection of infectious bronchitis virus. Diss. (sammanfattning/summary) Uppsala : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae, 1652-6880 ; 2013:2
ISBN 978-91-576-7759-4
[Doctoral thesis]

[img]
Preview
PDF
462kB

Abstract

This thesis deals with the molecular characterization and detection of infectious bronchitis virus (IBV), an important pathogen that causes heavy losses in the poultry populations worldwide. The aim of the research was to better understand the molecular characteristics of the virus and to investigate the factors behind the continuous emergence of new genetic variants and the occurrence of outbreaks. The studies showed that the viral genome is under a continuous evolution, due to mutations, strong selective pressure and recombination events. These forces lead to a wide genetic diversity and the generation of new variants of this virus. The viral genes encoding the spike, envelope and membrane proteins can be considered the main genomic regions, which are indicating the evolution processes of IBV. The various strains contain specific structural and functional motifs in their genes and the alterations in these motifs may affect the infection biology of the virus. The constant emergence of new variants in Sweden is likely due to the introduction of novel IBV strains from other European countries through the import of poultry products, or by the continuous migration of wild birds.

The in silico investigations of the spike glycoprotein coding regions of the Massachusetts and QX-like genotypes demonstrated molecular differences between these genotypic variants. It is hypothesized that the genetic diversity in the spike gene of IBV and of other avian coronaviruses, as well as of human, bat, and other animal coronaviruses could be associated with the adaptation and host specificity of these infectious agents.

The data obtained by molecular characterization approach was also used for the development of a new molecular method for the improved detection and genotyping of this virus. A microarray platform was developed for the simultaneous detection and rapid typing of IBV variants. This assay provides a practical tool for better diagnosis, for studying the effectiveness of vaccination and for performing large-scale epidemiological studies.

Authors/Creators:Abro, Shahid Hussain
Title:Molecular characterization and detection of infectious bronchitis virus
Series/Journal:Acta Universitatis agriculturae Sueciae (1652-6880)
Year of publishing :6 December 2012
Volume:2013:2
Number of Pages:54
Papers/manuscripts:
NumberReferences
I.Abro S. H., Renström, L. H. M., Ullman, K., Isaksson, M., Zohari, S., Jansson, D. S., Belák, S., and Baule C. (2012). Emergence of novel strains of avian infectious bronchitis virus in Sweden. Veterinary Microbiology 155: 237–246.
II.Abro, S. H., Renström, L. H. M., Ullman, K., Belák, S. and Baule, C. (2012). Characterization and analysis of the full-length genome of a strain of the European QX-like genotype of infectious bronchitis virus. Archives of Virology 157: 1211–1215.
III.Abro, S. H., Ullman, K., Belák, S. and Baule, C. (2012). Bioinformatics and evolutionary insight on the spike glycoprotein gene of QX-like and Massachusetts strains of infectious bronchitis virus. Virology Journal 9:211.
IV.Öhrmalm, C., Abro, S. H., Baule, C., Zohari, S., Bálint, A., Renström, L. H. M., Blomberg, J. and Belák, S. Variation-tolerant capture multiplex assay (VOCMA) for the simultaneous detection of avian infectious bronchitis virus genotypes using multiplex RT-PCR and Luminex technology (Manuscript).
Place of Publication:Uppsala
Publisher:Dept. of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural Sciences
ISBN for printed version:978-91-576-7759-4
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Agris subject categories.:L Animal production > L73 Animal diseases
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Pathobiology
Agrovoc terms:avian infectious bronchitis virus, poultry, animal diseases, glycoproteins, mutation, genetic variation, data analysis, bioinformatics, genotypes, serotypes, immunological techniques, pcr
Keywords:Infectious bronchitis virus, IBV, coronavirus, spike glycoprotein, mutation, selective pressure, recombination, genetic diversity, bioinformatics, multiplex, diagnosis, VOCMA, microarray
URN:NBN:urn:nbn:se:slu:epsilon-e-761
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-761
ID Code:9299
Department:(VH) > Dept. of Biomedical Sciences and Veterinary Public Health
Deposited By: Mr shahid abro
Deposited On:10 Dec 2012 08:03
Metadata Last Modified:02 Dec 2014 10:53

Repository Staff Only: item control page

Downloads

Downloads per year (since September 2012)

View more statistics

Downloads
Hits