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Olfactory mechanisms of host selection in phytophagous insects

behavior, neuron, and receptor

Binyameen, Muhammad (2013). Olfactory mechanisms of host selection in phytophagous insects. Diss. (sammanfattning/summary) Alnarp : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae, 1652-6880 ; 2013:11
ISBN 978-91-576-7768-6
[Doctoral thesis]

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Abstract

The most challenging tasks for phytophagous insects are the location and selection of mates, food sources, and oviposition sites, all crucial for survival and reproduction. To perform these tasks insects rely largely on their sense of smell (olfaction). I address how the insect olfactory system discriminates between components of complex odor mixtures, modulating behavior and fitness. I have studied modulation of attraction in the moth Spodoptera littoralis and the bark beetle Ips typographus by separation of pheromone (Ph) and anti-attractants, and of Ph components alone. An antagonist reduced male moth attraction towards the female sex Ph, and a blend of non-host volatiles (NHV) reduced attraction of both sexes of I. typographus towards their Ph, insect catches decreased with decreasing odor-source distance. Conversely, increasing distance between Ph components decreased attraction in both insect species. However, moths were more sensitive to small-scale spacing. Reproductive behaviors as well as fecundity and longevity of S. littoralis moths were negatively affected in the presence of volatiles from leaves of non-host plants, Picea abies or Adhatoda vasica. The presence of non-host plants strongly modulated male moths’ behavior, reducing their attraction towards the Ph source in flight assays. Gas chromatography-electroantenno-graphic detection (GC-EAD) by female S. littoralis antennae with headspace volatile collections from P. abies and A. vasica revealed eight active compounds, with seven new actives. Single sensillum recordings (SSR) created a functional-morphological map of 49 olfactory sensory neuron (OSN) functional types in six morphological sensillum types in female S. littoralis. Proximally located OSNs showed a higher sensitivity, shorter latency, and displayed more phasic responses than distally located OSNs of the same class. GC-SSRs with volatiles from a larval host, cotton plants, and the adult nectar source, lilac flowers, revealed 38 active compounds for female OSNs, including 12 new actives. The odor response specificities of four olfactory receptor (OR) genes of S. littoralis were deorphanized by expression in the Empty Neuron System (ENS) of Drosophila melanogaster using SSR and GC-SSR (GC-SSR-ENS). Two of the ORs responded specifically to single odorants, while the other two responded similarly to the same 9 compounds, but dose-response experiments with new compounds, identified by GC-SSR, revealed specific odor-response profiles.

Authors/Creators:Binyameen, Muhammad
Title:Olfactory mechanisms of host selection in phytophagous insects
Subtitle:behavior, neuron, and receptor
Series/Journal:Acta Universitatis agriculturae Sueciae (1652-6880)
Year of publishing :15 February 2013
Volume:2013:11
Number of Pages:57
Papers/manuscripts:
NumberReferences
IAndersson MN, Binyameen M, Sadek MM, Schlyter F (2011). Attraction Modulated by Spacing of Pheromone Components and Anti-attractants in a Bark Beetle and a Moth. Journal of Chemical Ecology 37(8), 899-911.
IIBinyameen M, Hussain A, Yousefi F, Birgersson G, Schlyter F. Modulation of the Reproductive Behaviors by Non-host Plant Volatiles in the Polyphagous Egyptian Cotton Leafworm, Spodoptera littoralis. Submitted.
IIIBinyameen M, Anderson P, Ignell R, Seada MA, Hansson BS, Schlyter F (2012). Spatial Organization of Antennal Olfactory Sensory Neurons in the Female Spodoptera littoralis Moth: Differences in Sensitivity and Temporal Characteristics. Chemical Senses 37(7), 613-629
IVBinyameen M, Hansson BS, Birgersson G, Schlyter F. Identification of New Semiochemicals for Spodoptera littoralis from Induced Green Plants and Flowers using GC-SSR and GC-MS. Manuscript.
VBinyameen M, Walker WB III, Montagné N, Chertemps T, Jacquin-Joly E, Schlyter F, Anderson P, Hansson BS, Larsson MC. Deorphanization of Olfactory Receptors Tuned to Host Volatile by Heterologous Expression in Drosophila Empty Neuron System, SSR, and GC-SSR-ENS in a Moth. Manuscript
Place of Publication:Alnarp
Publisher:Dept. of Plant Protection Biology, Swedish University of Agricultural Sciences
ISBN for printed version:978-91-576-7768-6
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Subjects:Obsolete subject words > NATURAL SCIENCES
Obsolete subject words > NATURAL SCIENCES > Biology
Obsolete subject words > NATURAL SCIENCES > Biology > Organism biology
Obsolete subject words > NATURAL SCIENCES > Biology > Organism biology > Plant physiology
Agrovoc terms:olfactory organs, host plants, sensory receptors, pest insects, spodoptera littoralis, ips typographus
Keywords:Spodoptera littoralis, Ips typographus, olfaction, olfactory sensory neuron, olfactory receptor, single sensillum recordings, plant volatiles, non-host volatiles
URN:NBN:urn:nbn:se:slu:epsilon-e-874
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-874
ID Code:9440
Department:(LTJ, LTV) > Department of Plant Protection Biology
External funders:FORMAS
Deposited By: Mr. Muhammad Binyameen
Deposited On:18 Feb 2013 10:15
Metadata Last Modified:14 Dec 2014 04:34

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