Borén, Mats
(2005).
Proteomics of barley starch granules.
Diss. (sammanfattning/summary)
Uppsala :
Sveriges lantbruksuniv.,
Acta Universitatis Agriculturae Sueciae, 1652-6880
; 2005:107
ISBN 91-576-6909-6
[Doctoral thesis]
![]() |
PDF
10MB |
Abstract
Starches in various forms are an important part of the human diet. Furthermore, the use of starch as a renewable and biodegradable raw material for various industrial applications is becoming increasingly attractive. Non-food applications of starch make new demands on quality and type of starches produced. Ideally these new demands should be met by tailoring starches within the plant. A number of such in planta produced specialty starches exist today, e.g. high-amylose starch. Our aims with this Ph.D. project have been to increase the knowledge of starch synthesis and caryopsis development. We have attempted to do this primarily by investigating the proteins entrapped within the starch granule. This has been done using proteomic techniques. Our main focus has been on characterizing a barley mutant with high amylose starch, amo1. Apart from proteomic characterization of granule proteins we have also done comparative starch characterizations and assayed for starch branching activity in mutant and wild type seeds. We also investigated the occurrence of a caspase-like protease activity, a VEIDase, during caryopsis development. A protocol for extracting proteins from starch granules has been developed and a 2D proteomic reference map of integral granule proteins established. The molecular mechanism of a fragmentation of granule proteins induced during sample preparation has been found. An alternative sample preparation procedure has been established that reduces fragmentation considerably. A developmentally regulated caspase-like proteolytic activity, a VEIDase, with possible connection to developmental programmed cell death, has been characterized and found to be chiefly active during early stages of caryopsis development. The VEIDase activity has been localised to small vesicles in starchy endosperm cells.
Authors/Creators: | Borén, Mats | ||||
---|---|---|---|---|---|
Title: | Proteomics of barley starch granules | ||||
Series Name/Journal: | Acta Universitatis Agriculturae Sueciae | ||||
Year of publishing : | November 2005 | ||||
Number: | 2005:107 | ||||
Number of Pages: | 42 | ||||
Papers/manuscripts: |
| ||||
Place of Publication: | Uppsala | ||||
ISBN for printed version: | 91-576-6909-6 | ||||
ISSN: | 1652-6880 | ||||
Language: | English | ||||
Publication Type: | Doctoral thesis | ||||
Full Text Status: | Public | ||||
Agris subject categories.: | F Plant production > F60 Plant physiology and biochemistry | ||||
Subjects: | Not in use, please see Agris categories | ||||
Agrovoc terms: | barley, starch, granules, proteins, hydrolysis, seeds, endosperm | ||||
Keywords: | barley, starch, endosperm, starch granule, proteomics, protein, 2D PAGE, VEIDase, developmental PCD, acid induced hydrolysis, Asp-Pro, fragmentation | ||||
URN:NBN: | urn:nbn:se:slu:epsilon-797 | ||||
Permanent URL: | http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-797 | ||||
ID Code: | 974 | ||||
Department: | (NL, NJ) > Dept. of Plant Biology and Forest Genetics (until 131231) | ||||
Deposited By: | Mats Borén | ||||
Deposited On: | 09 Nov 2005 00:00 | ||||
Metadata Last Modified: | 02 Dec 2014 10:08 |
Repository Staff Only: item control page