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Proteomics of barley starch granules

Borén, Mats (2005). Proteomics of barley starch granules. Diss. (sammanfattning/summary) Uppsala : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae, 1652-6880 ; 2005:107
ISBN 91-576-6909-6
[Doctoral thesis]

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Abstract

Starches in various forms are an important part of the human diet. Furthermore, the use of starch as a renewable and biodegradable raw material for various industrial applications is becoming increasingly attractive. Non-food applications of starch make new demands on quality and type of starches produced. Ideally these new demands should be met by tailoring starches within the plant. A number of such in planta produced specialty starches exist today, e.g. high-amylose starch. Our aims with this Ph.D. project have been to increase the knowledge of starch synthesis and caryopsis development. We have attempted to do this primarily by investigating the proteins entrapped within the starch granule. This has been done using proteomic techniques. Our main focus has been on characterizing a barley mutant with high amylose starch, amo1. Apart from proteomic characterization of granule proteins we have also done comparative starch characterizations and assayed for starch branching activity in mutant and wild type seeds. We also investigated the occurrence of a caspase-like protease activity, a VEIDase, during caryopsis development. A protocol for extracting proteins from starch granules has been developed and a 2D proteomic reference map of integral granule proteins established. The molecular mechanism of a fragmentation of granule proteins induced during sample preparation has been found. An alternative sample preparation procedure has been established that reduces fragmentation considerably. A developmentally regulated caspase-like proteolytic activity, a VEIDase, with possible connection to developmental programmed cell death, has been characterized and found to be chiefly active during early stages of caryopsis development. The VEIDase activity has been localised to small vesicles in starchy endosperm cells.

Authors/Creators:Borén, Mats
Title:Proteomics of barley starch granules
Year of publishing :November 2005
Volume:2005:107
Number of Pages:42
Papers/manuscripts:
NumberReferences
ALLI. Borén, M., Larson, H., Falk, A., Jansson, C. 2004. The barley starch granule proteome – internalized granule polypeptides of the mature endosperm. Plant Science 166, 617-626. II. Borén, M., Bozhkov, P., Höglund, A., Falk, A., Jansson, C. 2005 Developmental regulation if a VEIDase caspase-like proteolytic activity in barley caryopsis. (under revision in J. Exp. Botany) III. Borén, M., Larson, H., Falk, A., Jansson, C. 2005 Acid hydrolysis causes fragmentation of intrinsic starch granule proteins during extraction. (submitted) IV. Borén, M., Glaring, M., Ghebremedhin, H., Blenow, A., Falk, A., Jansson, C. Molecular Characterisation of the High Amylose Barley Mutant amo1. (manuscript)
Place of Publication:Uppsala
ISBN for printed version:91-576-6909-6
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Agris subject categories.:F Plant production > F60 Plant physiology and biochemistry
Subjects:Not in use, please see Agris categories
Agrovoc terms:barley, starch, granules, proteins, hydrolysis, seeds, endosperm
Keywords:barley, starch, endosperm, starch granule, proteomics, protein, 2D PAGE, VEIDase, developmental PCD, acid induced hydrolysis, Asp-Pro, fragmentation
URN:NBN:urn:nbn:se:slu:epsilon-797
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-797
ID Code:974
Department:(NL, NJ) > Dept. of Plant Biology and Forest Genetics (until 131231)
Deposited By: Mats Borén
Deposited On:09 Nov 2005 00:00
Metadata Last Modified:02 Dec 2014 10:08

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