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Epidemiology, detection and prevention of respiratory virus infections in Swedish cattle

with special reference to bovine respiratory syncytial virus

Hägglund, Sara (2005). Epidemiology, detection and prevention of respiratory virus infections in Swedish cattle. Diss. (sammanfattning/summary) Uppsala : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae, 1652-6880 ; 2005:121
ISBN 91-576-6920-1
[Doctoral thesis]

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Abstract

This thesis deals with the dynamics of bovine respiratory syncytial virus (BRSV), bovine parainfluenza virus 3 (PIV-3) and bovine coronavirus (BCoV) infections in Swedish cattle herds. It also describes the development of a fluorogenic reverse transcription (fRT)-PCR for BRSV diagnosis. Furthermore, BRSV immunostimulating complexes (ISCOMs) and a commercial inactivated BRSV vaccine were evaluated in vivo in calves. By serology, high incidences of PIV-3 and BCoV, and a lower incidence of BRSV, were demonstrated in 118 herds in one area of Sweden and at a Swedish bull testing station for beef breeds. In the dairy herds, absence of these infections and bovine viral diarrhea virus (BVDV) was correlated to absence of respiratory disease, confirming the clinical importance of the viruses. There appeared to be a geographical clustering of BRSV and BCoV infected herds and infections were as common in herds that did or did not introduce new animals, indicating indirect transmission between herds. At the bull testing station, an effective spread of virus within the herd was demonstrated. BRSV was involved in a disease outbreak, emphasizing the need for effective means to prevent this infection. By testing animals prior to admission, the bull testing station was kept free from BVDV during a six-year study period. A single tube BRSV fRT-PCR was shown to be a sensitive, specific and rapid tool to detect BRSV in both naturally and experimentally infected animals. The assay required less laboratory time, had a lower risk of contamination and allowed higher sample throughput than a nested PCR with a comparable sensitivity. Accordingly, the fRT-PCR is suitable for BRSV research as well as for routine diagnostic purposes and was used in the vaccine evaluation. The immunogenicity and protection induced by BRSV-ISCOMs were evaluated and compared to those induced by a commercial vaccine (CV) in calves. An experimental BRSV challenge was used for this purpose. The ISCOMs induced rapid humoral immune responses and strong clinical and virological protection despite maternal antibodies in calves at vaccination. In contrast, calves vaccinated with the CV and control calves all shed virus and developed moderate to severe respiratory disease after challenge. Thus, BRSV-ISCOMs are promising candidates for future industrial production after further evaluation and may also be used to investigate protective immunological parameters against BRSV. The CV needs to be further evaluated in controlled field trials.

Authors/Creators:Hägglund, Sara
Title:Epidemiology, detection and prevention of respiratory virus infections in Swedish cattle
Subtitle:with special reference to bovine respiratory syncytial virus
Year of publishing :November 2005
Volume:2005:121
Number of Pages:59
Papers/manuscripts:
NumberReferences
ALLI. Hägglund, S., Svensson, C., Emanuelson, U., Valarcher, J.F. & Alenius, S. 2005. Dynamics of virus infections involved in the bovine respiratory disease complex in Swedish dairy herds. The Veterinary Journal. In press. II. Hägglund, S., Hjort, M., Öhagen, P., Törnqvist, M. & Alenius, S. A six-year study on respiratory viral infections in beef calves. Submitted. III. Hakhverdyan, M., Hägglund, S., Larsen, L.E. & Belák, S. 2005. Evaluation of a single-tube fluorogenic RT-PCR assay for detection of bovine respiratory syncytial virus in clinical samples. Journal of Virological Methods 123, 195-202. IV. Hägglund, S., Hu, K.F., Larsen, L.E., Hakhverdyan, M., Valarcher, J.F., Taylor, G., Morein, B., Belák, S. & Alenius, S. 2004. Bovine respiratory syncytial virus ISCOMs--protection in the presence of maternal antibodies. Vaccine 23, 646-655.
Place of Publication:Uppsala
ISBN for printed version:91-576-6920-1
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Agris subject categories.:L Animal production > L73 Animal diseases
Subjects:Not in use, please see Agris categories
Agrovoc terms:cattle, herds, bovine respiratory syncytial virus, diarrhoea, viroses, coronaviridae, paramyxoviridae, respiratory diseases, animal diseases, disease transmission, pcr, laboratory diagnosis, sweden
Keywords:cattle, BRSV, PIV-3, BCoV, BCV, BVDV, respiratory disease, PCR, ISCOM, vaccine
URN:NBN:urn:nbn:se:slu:epsilon-821
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-821
ID Code:986
Department:(VH) > Dept. of Clinical Sciences
Deposited By: Sara Hägglund
Deposited On:28 Nov 2005 00:00
Metadata Last Modified:02 Dec 2014 10:08

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