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Disruption of the Eng18B ENGase gene in the fungal biocontrol agent trichoderm atroviride affetcs growth conidation and antagonistic ability

Sandgren, Mats and Dubey, Mukesh and Jensen, Dan Funck and Karlsson, Magnus and Wimal, Ubhayasekera (2012). Disruption of the Eng18B ENGase gene in the fungal biocontrol agent trichoderm atroviride affetcs growth conidation and antagonistic ability. PloS one. 7:5, 1-11
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Official URL: http://dx.doi.org/10.1371/journal.pone.0036152

Abstract

The recently identified phylogenetic subgroup B5 of fungal glycoside hydrolase family 18 genes encodes enzymes with mannosyl glycoprotein endo-N-acetyl-b-D-glucosaminidase (ENGase)-type activity. Intracellular ENGase activity is associated with the endoplasmic reticulum associated protein degradation pathway (ERAD) of misfolded glycoproteins, although the biological relevance in filamentous fungi is not known. Trichoderma atroviride is a mycoparasitic fungus that is used for biological control of plant pathogenic fungi. The present work is a functional study of the T. atroviride B5-group gene Eng18B, with emphasis on its role in fungal growth and antagonism. A homology model of T. atroviride Eng18B structure predicts a typical glycoside hydrolase family 18 (ab) 8 barrel architecture. Gene expression analysis shows that Eng18B is induced in dual cultures with the fungal plant pathogens Botrytis cinerea and Rhizoctonia solani, although a basal expression is observed in all growth conditions tested. Eng18B disruption strains had significantly reduced growth rates but higher conidiation rates compared to the wild-type strain. However, growth rates on abiotic stress media were significantly higher in Eng18B disruption strains compared to the wild-type strain. No difference in spore germination, germ-tube morphology or in hyphal branching was detected. Disruption strains produced less biomass in liquid cultures than the wild-type strain when grown with chitin as the sole carbon source. In addition, we determined that Eng18B is required for the antagonistic ability of T. atroviride against the grey mould fungus B. cinerea in dual cultures and that this reduction in antagonistic ability is partly connected to a secreted factor. The phenotypes were recovered by re-introduction of an intact Eng18B gene fragment in mutant strains. A putative role of Eng18B ENGase activity in the endoplasmic reticulum associated protein degradation pathway of endogenous glycoproteins in T. atroviride is discussed in relation to the observed phenotypes.

Authors/Creators:Sandgren, Mats and Dubey, Mukesh and Jensen, Dan Funck and Karlsson, Magnus and Wimal, Ubhayasekera
Title:Disruption of the Eng18B ENGase gene in the fungal biocontrol agent trichoderm atroviride affetcs growth conidation and antagonistic ability
Series/Journal:PloS one (1932-6203)
Year of publishing :2012
Volume:7
Number:5
Page range:1-11
Number of Pages:11
Publisher:Public Library of Science
ISSN:1932-6203
Language:English
Publication Type:Journal article
Refereed:Yes
Article category:Scientific peer reviewed
Version:Published version
Full Text Status:Public
Agris subject categories.:X Agricola extesions > X30 Life sciences
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Microbiology (Microbiology in the medical area to be 30109)
Keywords:Trichoderma atroviride, Gene expression analysis, Disruption strains
URN:NBN:urn:nbn:se:slu:epsilon-e-1493
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-1493
Additional ID:
Type of IDID
Web of Science (WoS)000305335000025
DOI10.1371/journal.pone.0036152
ID Code:10329
Department:(NL, NJ) > Dept. of Microbiology (until 161231)
(NL, NJ) > Dept. of Forest Mycology and Plant Pathology
(S) > Dept. of Forest Mycology and Plant Pathology
Deposited By: SLUpub Connector
Deposited On:14 Jun 2013 12:00
Metadata Last Modified:29 Jan 2015 23:16

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