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Novel insights into the action of SHI/STY transcriptional regulators during plant development

Cierlik, Izabela (2014). Novel insights into the action of SHI/STY transcriptional regulators during plant development. Diss. (sammanfattning/summary) Uppsala : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae, 1652-6880 ; 2014:13
ISBN 978-91-576-7974-1
eISBN 978-91-576-7975-8
[Doctoral thesis]

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Abstract

One key player in plant organogenesis is the phytohormone auxin, and this thesis reveal that members of the SHI/STY gene family participate in the regulation of auxin homeostasis and organ development in Arabidopsis thaliana.

Using inducible constructs, ChIP, and EMSA, we could show that the SHI/STY members act as transcriptional activators directly binding to the promoter of the auxin biosynthesis gene YUC4. Additional putative downstream targets were identified encoding transcription factors, other auxin biosynthesis enzymes and enzymes involved in cell wall modulations. This work also brings novel insight into SHI/STY-dependent regulation of cotyledon and leaf vascular patterning and stamen number. Detailed expression studies of SHI/STY genes and several downstream targets confirm their overlapping spatial and temporal expression pattern during cotyledon, leaf, stamen and gynoecium development, clearly supporting the partially redundant function of the SHI/STY family members during the development of these organs.

In addition, the expression of SHI/STY members is partially mediated through a conserved motif in their promoter region, a GCC-box, which appears to be essential for SHI/STY expression in aerial organs. Furthermore, a group of putative upstream regulators belonging to the AP2/ERF family have been identified, which requires the GCC-box for their transcriptional regulation of the SHI/STY genes.

Authors/Creators:Cierlik, Izabela
Title:Novel insights into the action of SHI/STY transcriptional regulators during plant development
Series/Journal:Acta Universitatis agriculturae Sueciae (1652-6880)
Year of publishing :2014
Volume:2014:13
Number of Pages:82
Papers/manuscripts:
NumberReferences
I.Eklund DM, Ståldal V, Valsecchi I, Cierlik I, Eriksson C, Hiratsu K, Ohme-Takagi M, Sundström JF, Thelander M, Ezcurra I, Sundberg E (2010). The Arabidopsis thaliana STYLISH1 protein acts as a transcriptional activator regulating auxin biosynthesis. Plant Cell 22(2), 349-363.
II.Ståldal V, Cierlik I, Chen S, Landberg K, Baylis T, Myrenås M, Sundström JF, Eklund DM, Ljung K, Sundberg E (2012). The Arabidopsis thaliana transcriptional activator STYLISH1 regulates genes affecting stamen development, cell expansion and timing of flowering. Plant Molecular Biology 78(6), 545-559.
III.Cierlik I, Landberg K, Claes A, Sundberg E. The Arabidopsis SHORT INTERNODES/STYLISH gene STY1 affects the activity of, and overlaps with, gene affecting cell expansion and metabolic processes (manuscript).
IV.Baylis T, Cierlik I, Sundberg E, Mattsson J (2013). SHORT INTERNODES/STYLISH genes, regulators of auxin homeostasis, are involved in leaf vein development in Arabidopsis thaliana. New Phytologist 197(3), 737-50.
V.Eklund DM*, Cierlik I*, Ståldal V, Claes AR, Vestman D, Chandler J, Sundberg E (2011). Expression of Arabidopsis SHORT INTERNODES/STYLISH family genes in auxin biosynthesis zones of aerial organs is dependent on a GCC box-like regulatory element. Plant Physiology 157(4), 2069-80.
UNSPECIFIED* Indicates shared first authorship
Place of Publication:Uppsala
Publisher:Dept. of Plant Biology, Swedish University of Agricultural Sciences
ISBN for printed version:978-91-576-7974-1
ISBN for electronic version:978-91-576-7975-8
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Agris subject categories.:F Plant production > F30 Plant genetics and breeding
F Plant production > F62 Plant physiology - Growth and development
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Developmental Biology
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 404 Agricultural Biotechnology > Genetics and Breeding
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 405 Other Agricultural Sciences > Environmental Sciences related to Agriculture and Land-use
Agrovoc terms:arabidopsis thaliana, plant reproductive organs, plant developmental stages, transcription, transcription factors, genes, gene expression, auxins, biosynthesis, homeostasis, biological development
Keywords:Arabidopsis thaliana, organ development, transcriptional regulation, SHI/STY family, STYLISH1
URN:NBN:urn:nbn:se:slu:epsilon-e-1777
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-1777
ID Code:10998
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Dept. of Plant Biology and Forest Genetics (until 131231)
External funders:FORMAS
Deposited By: Izabela Cierlik
Deposited On:07 Feb 2014 11:12
Metadata Last Modified:02 Dec 2014 11:04

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