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Pathogenic Escherichia coli and lipopolysaccharide enhance the expression of IL-8, CXCL5, and CXCL10 in canine endometrial stromal cells

Karlsson, Iulia and Hagman, Ragnvi and Guo, Yongzhi and Humblot, Patrice and Wang, Liya and Wernersson, Sara (2015). Pathogenic Escherichia coli and lipopolysaccharide enhance the expression of IL-8, CXCL5, and CXCL10 in canine endometrial stromal cells. Theriogenology. 84:1, 34-42
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Official URL: http://dx.doi.org/10.1016/j.theriogenology.2015.02...

Abstract

Chemokines play a central role in cellular communication in response to bacterial infection. However, the knowledge of the chemokine responses to bacterial infections in dogs remains limited. Uterine bacterial infection (pyometra) is one of the most common bacterial diseases in dogs and causes sepsis in most of the cases. We have shown previously that dogs with pyometra have higher messenger RNA (mRNA) levels of chemokines in uterus. To assess whether the stromal part of the endometrium expresses chemokines in response to bacterial infection, we cultured endometrial stromal cells isolated from healthy dogs and exposed them to either live pathogenic Escherichia coli, isolated from the uterus of a dog with pyometra, or lipopolysaccharide. Changes in the mRNA expression of ELR+ CXC chemokines, IL-8, CXCL5, CXCL7, and ELR- CXC chemokine, CXCL10, were measured after 24 hours using quantitative real-time polymerase chain reaction. Levels of IL-8, CXCL5, and CXCL10 were upregulated in endometrial stromal cells exposed to E coli and lipopolysaccharide, whereas the level of CXCL7 was decreased or unaffected. In addition, levels of IL-8 and CXCL5, but not CXCL7 or CXCL10, were significantly higher in dogs with pyometra than those in healthy dogs. Our findings show that pathogenic uterine-derived E coli induces a CXC chemokine response both in cultured endometrial stromal cells within 24 hours and in pyometra-affected uteri from dogs. Stromal cells could therefore play an important role in early neutrophil and T cell recruitment to the site of inflammation during gram-negative bacterial infection of the uterus. Further studies are needed to clarify the role of chemokines in host response to bacterial infection in dogs and the possibility of using chemokines as diagnostic parameters for bacterial infection in this species. (C) 2015 Elsevier Inc. All rights reserved.

Authors/Creators:Karlsson, Iulia and Hagman, Ragnvi and Guo, Yongzhi and Humblot, Patrice and Wang, Liya and Wernersson, Sara
Title:Pathogenic Escherichia coli and lipopolysaccharide enhance the expression of IL-8, CXCL5, and CXCL10 in canine endometrial stromal cells
Series/Journal:Theriogenology (0093-691X)
Year of publishing :2015
Depositing date:7 December 2015
Volume:84
Number:1
Page range:34-42
Number of Pages:9
Publisher:Elsevier
ISSN:0093-691X
Language:English
Publication Type:Journal article
Refereed:Yes
Article category:Scientific peer reviewed
Version:Accepted version
Full Text Status:Public
Agris subject categories.:L Animal production > L53 Animal physiology - Reproduction
L Animal production > L73 Animal diseases
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Medical Bioscience
Agrovoc terms:infection, cytokines, Endometrium, dogs, pyometra
Keywords:Infection, Chemokine, Endometrium, Dog/canine, Pyometra
URN:NBN:urn:nbn:se:slu:epsilon-e-3116
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-3116
Additional ID:
Type of IDID
Web of Science (WoS)000355572900005
DOI10.1016/j.theriogenology.2015.02.008
ID Code:12805
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Dept. of Clinical Sciences
(VH) > Dept. of Anatomy, Physiology and Biochemistry
Deposited By: SLUpub Connector
Deposited On:07 Dec 2015 14:11
Metadata Last Modified:13 Feb 2016 18:20

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