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Chromogranin a epitopes catestatin and vasostatin

evaluation of their potential use as clinical biomarkers for psychological and pain-induced stress in dogs

Srithunyarat, Thanikul (2017). Chromogranin a epitopes catestatin and vasostatin. Diss. (sammanfattning/summary) Uppsala : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae, 1652-6880 ; 2017:17
ISBN 978-91-576-8807-1
eISBN 978-91-576-8808-8
[Doctoral thesis]

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Abstract

Psychological and physiological factors, including pain, can induce a stress response. All today’s available methods for evaluating stress in dogs have shortcomings and it is therefore necessary to identify and evaluate new biomarkers. Chromogranin A (CgA) is a useful biomarker for stress assessment in humans. In dogs, the CgA epitopes catestatin (CST) and vasostatin (VS) can be measured. This thesis aimed to evaluate the potential use of the CgA epitopes CST and VS as biomarkers for psychological and pain-induced stress in dogs in a clinical setting.

Reference ranges of plasma CST, VS, and saliva CST concentrations in healthy low-stressed dogs were determined. Age, gender, breed, and time of day did not significantly affect CST and VS concentrations. CST and VS were evaluated as biomarkers for psychological stress. CST and VS in associated with other stress evaluation methods were compared in healthy dogs where one group was stressed and the other was not. In the stress group, saliva CST, serum cortisol, and stress scores increased significantly and saliva CST did not overlap with the reference range. Plasma CST and VS did not change significantly. CST and VS were further tested as biomarkers for pain-induced stress by comparing the concentrations in different surgical settings. CST, VS, and other pain and stress monitoring methods were investigated in healthy dogs that received analgesia and were subjected to elective ovariohysterectomy. Compared with before surgery, plasma CST decreased significantly during anesthetic recovery and at recall for suture removal and serum cortisol decreased significantly at recall, suggesting that CST may be a possible pain-induced stress biomarker. CST and VS were measured in dogs with fractures prior to and after morphine treatment and evaluated in association with other pain and stress evaluation methods and compared with control dogs. Circulating CST and cortisol decreased significantly in dogs with fractures, but did not differ significantly between before and after morphine analgesia. Plasma CST overlapped with the reference ranges but plasma VS did not differ significantly throughout the studies.

In conclusion, saliva CST may have potential as a psychological stress biomarker in dogs. Repeated sampling of plasma CST may be of interest for evaluating pain within the same patient. Plasma VS has no potential as a stress biomarker in dogs.

Authors/Creators:Srithunyarat, Thanikul
Title:Chromogranin a epitopes catestatin and vasostatin
Subtitle:evaluation of their potential use as clinical biomarkers for psychological and pain-induced stress in dogs
Alternative abstract:
LanguageAbstract
Swedish

Syftet med denna avhandling var att utvärdera om catestatin (CST) och vasostatin (VS) kan användas som biomarkörer för psykisk och smärtinducerad stress hos hundar.

Referensvärden för CST och VS i plasma och CST i saliv hos friska hundar med låg stressnivå bestämdes. Resultatet visade att ålder, kön, ras, och tid på dagen för provinsamlingen inte hade någon signifikant påverkan på CST och VS vare sig i plasma eller saliv. CST och VS potential som biomarkörer för psykisk stress utvärderades genom att jämföra koncentrationerna hos friska hundar med låg stressprofil med friska hundar med hög stressprofil. Hos hundarna med hög stressprofil uppmättes signifikant högre koncentrationer av CST i saliv och kortisol i serum jämfört med hundarna med låg stressprofil. CST i saliv var också högre än fastställda referensvärden, men ingen signifikant skillnad kunde påvisas a VS eende koncentrationerna av CST och VS i plasma hos hundar med låg och hög stressprofil. CST och VS testades ytterligare som möjliga biomarkörer för smärt-inducerad stress genom att jämföra koncentrationer vid olika kirurgiska ingrepp. Koncentrationerna av CST och VS tillsammans med andra metoder för monitorering av smärta och stress utvärderades hos friska hundar före och efter ovariohysterektomi. Alla hundar fick smärtlindring i samband med operationerna. Resultaten visade att koncentrationen av CST i plasma var signifikant lägre efter avslutad operation jämfört med innan operationen. Koncentrationen var också fortsatt låg vid återbesöken för stygntagning liksom kortisol. Resultaten antyder att CST möjligen kan ha potential som en biomarkör för smärtinducerad stress hos hund. För att vidare utvärdera om smärta påverkar koncentrationerna av CST och VS mättes nivåerna hos en grupp friska hundar och en grupp hundar som drabbats av benfrakturer. Prover togs före och efter smärtlindring med morfin. Resultatet visade att cirkulerande koncentrationer av CST och kortisol var signifikant lägre hos hundar med benfrakturer jämfört med friska hundar, men ingen signifikant skillnad kunde ses i prover tagna före jämfört med prover tagna efter morfingivan. Ingen signifikant förändring av koncentrationerna av VS kunde uppmätas vid någon tidpunkt i studien.

Sammanfattningsvis tyder resultaten på att CST i saliv kan ha en viss potential som en biomarkör för psykisk stress hos hund. Upprepade mätningar av plasma CST kan möjligen användas för att evaluera smärtutveckling hos en enskild hund.

Series/Journal:Acta Universitatis agriculturae Sueciae (1652-6880)
Year of publishing :28 April 2017
Depositing date:30 March 2017
Volume:2017:17
Number of Pages:86
Papers/manuscripts:
NumberReferences
ISrithunyarat, T., Hagman, R., Höglund, O.V., Olsson, U., Stridsberg, M., Jitpean, S., Lagerstedt, A.S., Pettersson, A. (2017). Catestatin and vasostatin concentrations in healthy dogs. Acta Veterinaria Scandinavica 59 (1), p. 1.
IISrithunyarat, T., Höglund, O.V., Hagman, R., Olsson, U., Stridsberg, M., Lagerstedt, A.S., Pettersson, A. (2016). Catestatin, vasostatin, cortisol, temperature, heart rate, respiratory rate, scores of the short form of Glasgow composite measure pain scale and visual analog scale for stress and pain behavior in dogs before and after ovariohysterectomy. BMC Research Notes 9 (1), p. 381.
IIISrithunyarat, T., Hagman, R., Höglund, O.V., Stridsberg, M., Olsson, U., Hanson, J., Nonthakotr, C., Lagerstedt, A.S., Pettersson, A. (2017). Catestatin, vasostatin, cortisol, and pain assessments in dogs suffering from traumatic bone fractures. BMC Research Notes 10 (1), p. 129.
IVSrithunyarat, T.*, Hagman, R., Höglund, O.V., Stridsberg, M., Hanson, J., Lagerstedt, A.S., Pettersson, A. Catestatin, vasostatin, cortisol, and visual analog scale scoring for stress assessment in healthy dogs (submitted)
Place of Publication:Uppsala
Publisher:Department of Clinical Sciences, Swedish University of Agricultural Sciences
ISBN for printed version:978-91-576-8807-1
ISBN for electronic version:978-91-576-8808-8
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Agris subject categories.:L Animal production > L70 Veterinary science and hygiene - General aspects
L Animal production > L73 Animal diseases
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Clinical Science
Agrovoc terms:dogs, pain, stress, fractures, drugs, behaviour, disease control
Keywords:Biomarker, Bone Fracture, Catestatin, Dog, Pain, Stress, Surgery, Vasostatin
URN:NBN:urn:nbn:se:slu:epsilon-e-4074
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-4074
ID Code:14210
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Dept. of Clinical Sciences
External funders:SLU fund for internationalisation of postgraduate studies
Deposited By: Dr. Thanikul Srithunyarat
Deposited On:04 Apr 2017 10:04
Metadata Last Modified:04 Apr 2017 10:41

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