microbial approaches for enhanced efficiency
Liu, Tong
(2019).
Biogas production from lignocellulosic agricultural residues.
Diss. (sammanfattning/summary)
Uppsala :
Sveriges lantbruksuniv.,
Acta Universitatis Agriculturae Sueciae, 1652-6880
; 2019:5
ISBN 978-91-7760-328-3
eISBN 978-91-7760-329-0
[Doctoral thesis]
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Abstract
Methane, produced through microbial anaerobic digestion of various organic materials, is seen as a promising sustainable bioenergy source with the potential to reduce the current dependence on fossil fuels. Among organic materials, lignocellulosic materials, especially agriculture residues, are highly interesting due to high abundance and potential for methane production. However, low nutrient content and highly recalcitrant structure often limit process efficiency. This thesis presents the results of in-depth studies conducted in order to obtain new information about lignocellulose-degrading bacteria in biogas processes and to identify ways to enable more efficient biogas production.
Different biogas processes were investigated in terms of their overall microbial community (bacteria and archaea) and potential lignocellulose degraders. The results showed that the biogas processes differed with regard to overall microbial community and chemical composition, but also composition of the cellulose-degrading bacterial community. These differences significantly influenced the degradation efficiency of both cellulose and wheat straw in batch digestion systems and also performance during start-up of semi-continuous stirred tank reactor (CSTR) processes. A positive correlation was found between lignocellulose degradation efficiency and relative abundance of Clostridium cellulolyticum. Ammonia level in the inoculum was identified as the most significant factor potentially affecting microbial community structure and methane production from lignocellulosic materials. Microbial and chemical composition of the original inoculum sources also influenced long-term degradation of lignocellulose in CSTR and appeared to influence residual methane potential. Different molecular methods for microbial community analysis were explored, with the aim of building an appropriate pipeline for in-depth studies of lignocellulose degraders in anaerobic reactors.
This thesis provides novel information about the microbial communities involved in degradation of lignocellulosic materials and possible connections to process parameters. This information could potentially enable biogas production to be steered towards a more efficient and controllable process for degradation and biogas production from agriculture residues and plant-based materials.
Authors/Creators: | Liu, Tong | ||||||||||
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Title: | Biogas production from lignocellulosic agricultural residues | ||||||||||
Subtitle: | microbial approaches for enhanced efficiency | ||||||||||
Alternative abstract: |
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Series Name/Journal: | Acta Universitatis Agriculturae Sueciae | ||||||||||
Year of publishing : | 8 January 2019 | ||||||||||
Depositing date: | 8 January 2019 | ||||||||||
Number: | 2019:5 | ||||||||||
Number of Pages: | 71 | ||||||||||
Papers/manuscripts: |
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Place of Publication: | Uppsala | ||||||||||
Publisher: | Department of Molecular Sciences, Swedish University of Agricultural Sciences | ||||||||||
ISBN for printed version: | 978-91-7760-328-3 | ||||||||||
ISBN for electronic version: | 978-91-7760-329-0 | ||||||||||
ISSN: | 1652-6880 | ||||||||||
Language: | English | ||||||||||
Publication Type: | Doctoral thesis | ||||||||||
Full Text Status: | Public | ||||||||||
Agris subject categories.: | P Natural resources > P06 Renewable energy resources | ||||||||||
Subjects: | (A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Biochemistry and Molecular Biology (A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Microbiology (Microbiology in the medical area to be 30109) (A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Ecology (A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 405 Other Agricultural Sciences > Renewable Bioenergy Research | ||||||||||
Keywords: | anaerobic digestion, lignocellulose, hydrolase families 5 and 48, biomethane potential, continuous stirred-tank reactor, co-digestion, residual methane potential, next-generation amplicon sequencing, terminal restriction fragment length polymorphism (T-RFLP) | ||||||||||
URN:NBN: | urn:nbn:se:slu:epsilon-e-5160 | ||||||||||
Permanent URL: | http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-5160 | ||||||||||
ID Code: | 15820 | ||||||||||
Faculty: | NJ - Fakulteten för naturresurser och jordbruksvetenskap | ||||||||||
Department: | (NL, NJ) > Department of Molecular Sciences | ||||||||||
External funders: | China Scholarship Council | ||||||||||
Deposited By: | Tong Liu | ||||||||||
Deposited On: | 08 Jan 2019 09:24 | ||||||||||
Metadata Last Modified: | 09 Sep 2020 14:17 |
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