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Filter paper is a simple and cost-effective transport medium for serological diagnosis of Peste des petits ruminants

Torsson, Emeli and Abubakar, Muhammad and Alvåsen, Karin and Manzoor, Shumaila and Roos, Nils and Kgotlele, Tebogo and Bin Zahur, Aamer and Misinzo, Gerald and Berg, Mikael and Wensman, Jonas Johansson (2019). Filter paper is a simple and cost-effective transport medium for serological diagnosis of Peste des petits ruminants. Small Ruminant Research. 170 , 154-159
[Journal article]

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Abstract

Peste des petits ruminants (PPR) is a highly contagious disease caused by peste-des-petits-ruminants virus. Following the successful eradication of the related rinderpest virus, a program to control and eradicate PPR was launched by the FAO and OIE. PPR is today present in many tropical countries where maintaining the cold chain for sample transportation is one of the major barriers for timely processing. Transport of samples on filter paper is a simple and cost-effective method, however validation and optimization is required to fully adapt this approach. The objective of this study was to evaluate and validate the use of filter paper in serological diagnosis of PPR. Blood samples (serum and filter paper) were collected from sheep and goats in both Tanzania and Pakistan and analysed using a PPRV-specific cELISA. The positive proportion was 10.7% in Tanzania and 80% in Pakistan when performing the analysis on serum. These results were then considered as reference and compared to the results from the filter papers analysed by the same cELISA. According to the statistical analysis the cut-off for a positive results for samples stored on filter paper was adjusted from < 50 % competition percentage to < 84% in Tanzania and to < 69% in Pakistan.These results demonstrate that filter papers are an acceptable and cost-effective transport method of whole blood samples for later use in serological analysis.

Authors/Creators:Torsson, Emeli and Abubakar, Muhammad and Alvåsen, Karin and Manzoor, Shumaila and Roos, Nils and Kgotlele, Tebogo and Bin Zahur, Aamer and Misinzo, Gerald and Berg, Mikael and Wensman, Jonas Johansson
Title:Filter paper is a simple and cost-effective transport medium for serological diagnosis of Peste des petits ruminants
Year of publishing :2019
Volume:170
Page range:154-159
Number of Pages:6
Publisher:Elsevier
ISSN:0921-4488
Language:English
Publication Type:Journal article
Refereed:Yes
Article category:Scientific peer reviewed
Version:Accepted version
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Clinical Science
Keywords:Peste des petits ruminants, Serological diagnosis, Filter papers, Transport, Tanzania, Pakistan, PPR
URN:NBN:urn:nbn:se:slu:epsilon-p-98403
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-98403
Additional ID:
Type of IDID
DOI10.1016/j.smallrumres.2018.11.023
Web of Science (WoS)000457658800024
ID Code:16133
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Dept. of Clinical Sciences
(VH) > Dept. of Biomedical Sciences and Veterinary Public Health
Deposited By: SLUpub Connector
Deposited On:24 May 2019 09:26
Metadata Last Modified:29 Nov 2019 00:15

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