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Microbial inputs in coffee (Coffea arabica L.) production systems, southwestern Ethiopia

implications for promotion of biofertilizers and biocontrol agents

Muleta, Diriba (2007). Microbial inputs in coffee (Coffea arabica L.) production systems, southwestern Ethiopia. Diss. (sammanfattning/summary) Uppsala : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae, 1652-6880 ; 2007:117
ISBN 978-91-85913-16-9
[Doctoral thesis]

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Abstract

Arabica coffee is the key cash crop and top mainstay of the Ethiopian economy and requires sustainable production methods. Southwestern natural forests, the site of this study, are believed to be the centre of origin and diversity for Coffea arabica and still harbour wild Arabica coffee that may serve as an important gene pool for future breeding. Cost reductions, sustainability and quality improvement are now the major priorities in coffee production systems and require organic growing of coffee. Current developments in sustainability involve rational exploitation of soil microbial activities that positively affect plant growth and this study examines this possibility. The composition of coffee shade tree species and density of arbuscular mycorrhizal fungi (AMF) spores and coffee-associated rhizobacteria in different coffee production systems in southwestern Ethiopia were investigated. The main objectives were to: 1) systematically identify the dominant coffee shade tree species; 2) quantify and characterize AMF populations with respect to spatial distribution; 3) screen for beneficial rhizobacteria (microbial biofertilizers and biocontrol agents), particularly in the rhizosphere of coffee plants; and 4) characterize rhizobacterial isolates of particular interest using molecular tools (polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis and 16S rDNA gene sequencing). Sampling and determination of microbial functional characteristics followed standard methods. Nineteen dominant shade tree species belonging to 14 plant families were identified, with the tree legume (Millettia ferruginea) dominating. All soil samples contained AMF spores and members of the Glomeromycota, Glomus spp. dominating. AMF spore density was affected by sampling point, site, depth, shade tree species and shade tree/coffee plant age. Coffee-associated rhizobacterial isolates showed multiple beneficial traits (phosphate solubilization, production of organic acids, siderophores, indoleacetic acid, hydrogen cyanide, lytic enzymes and degradation of an ethylene precursor). Many isolates also revealed a potent inhibitory effect against emerging fungal coffee pathogens such as Fusarium xylarioides, F. stilboides and F. oxysporum. According to in vitro studies Bacillus, Erwinia, Ochrobactrum, Pseudomonas, and Serratia spp. were the most important isolates to act as potential biofertilizers, biocontrol agents or both. Thus, these indigenous isolates deserve particular attention and further greenhouse and field trials could ascertain their future applicability for inoculum development.

Authors/Creators:Muleta, Diriba
Title:Microbial inputs in coffee (Coffea arabica L.) production systems, southwestern Ethiopia
Subtitle:implications for promotion of biofertilizers and biocontrol agents
Year of publishing :2007
Volume:2007:117
Number of Pages:67
Papers/manuscripts:
NumberReferences
ALLI. Muleta, D., Assefa, F., Nemomissa, S. & Granhall, U. 2007. Composition of coffee shade tree species and density of indigenous arbuscular mycorrhizal fungi (AMF) spores in Bonga natural coffee forest, southwestern Ethiopia. Forest Ecology & Management 241, 145-154. II. Muleta, D., Assefa, F., Nemomissa, S. & Granhall, U. Distribution of arbuscular mycorrhizal fungi spores in soils of smallholder agroforestry and monocultural coffee systems in southwestern Ethiopia. Submitted III. Muleta, D., Assefa, F., Börjesson, E. & Granhall, U. Phosphate-solubilising rhizobacteria associated with Coffea arabica L. in natural coffee forests of southwestern Ethiopia. Submitted IV. Muleta, D., Assefa, F. & Granhall, U. 2007. In vitro antagonism of rhizobacteria isolated from Coffea arabica L. against emerging fungal coffee pathogens. Engineering in Life Sciences 7, 1-11. V. Muleta, D., Assefa, F., Hjort, K., Roos, S. & Granhall, U. Characterization of rhizobacteria isolated from wild Coffea arabica L. with emphasis on some plant growth promoting traits. Submitted
Place of Publication:Uppsala
ISBN for printed version:978-91-85913-16-9
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Agrovoc terms:coffea arabica, forest soils, shade plants, leguminosae, agroforestry, vesicular arbuscular mycorrhizae, rhizobacteria, biological control agents, biofertilizers, fusarium, pathogens, hydrocyanic acid, ethiopia
Keywords:ACC, fungal coffee pathogens, Glomeromycota, hydrogen cyanide, IAA, lytic enzymes, phosphobacteria, PGPR, siderophores, tree legumes
URN:NBN:urn:nbn:se:slu:epsilon-1965
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-1965
ID Code:1657
Department:(NL, NJ) > Dept. of Microbiology (until 161231)
Deposited By: Diriba Muleta
Deposited On:12 Dec 2007 00:00
Metadata Last Modified:02 Dec 2014 10:13

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