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RNA Interference and CRISPR/Cas Gene Editing for Crop Improvement: Paradigm Shift towards Sustainable Agriculture

Rajput, Meenakshi and Choudhary, Khushboo and Kumar, Manish and Vivekanand, V. and Chawade, Aakash and Ortiz, Rodomiro and Pareek, Nidhi (2021). RNA Interference and CRISPR/Cas Gene Editing for Crop Improvement: Paradigm Shift towards Sustainable Agriculture. Plants. 10 , 1914
[Article Review/Survey]

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Abstract

With the rapid population growth, there is an urgent need for innovative crop improvement approaches to meet the increasing demand for food. Classical crop improvement approaches involve, however, a backbreaking process that cannot equipoise with increasing crop demand. RNA-based approaches i.e., RNAi-mediated gene regulation and the site-specific nuclease-based CRISPR/Cas9 system for gene editing has made advances in the efficient targeted modification in many crops for the higher yield and resistance to diseases and different stresses. In functional genomics, RNA interference (RNAi) is a propitious gene regulatory approach that plays a significant role in crop improvement by permitting the downregulation of gene expression by small molecules of interfering RNA without affecting the expression of other genes. Gene editing technologies viz. the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein (CRISPR/Cas) have appeared prominently as a powerful tool for precise targeted modification of nearly all crops’ genome sequences to generate variation and accelerate breeding efforts. In this regard, the review highlights the diverse roles and applications of RNAi and CRISPR/Cas9 system as powerful technologies to improve agronomically important plants to enhance crop yields and increase tolerance to environmental stress (biotic or abiotic). Ultimately, these technologies can prove to be important in view of global food security and sustainable agriculture.

Authors/Creators:Rajput, Meenakshi and Choudhary, Khushboo and Kumar, Manish and Vivekanand, V. and Chawade, Aakash and Ortiz, Rodomiro and Pareek, Nidhi
Title:RNA Interference and CRISPR/Cas Gene Editing for Crop Improvement: Paradigm Shift towards Sustainable Agriculture
Series Name/Journal:Plants
Year of publishing :2021
Volume:10
Article number:1914
Number of Pages:31
Language:English
Publication Type:Article Review/Survey
Article category:Scientific peer reviewed
Version:Published version
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 404 Agricultural Biotechnology > Plant Biotechnology
Keywords:crop, CRISPR/Cas9, resistance, RNA interference, stress
URN:NBN:urn:nbn:se:slu:epsilon-p-113547
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-113547
Additional ID:
Type of IDID
DOI10.3390/plants10091914
Web of Science (WoS)000700526000001
Otherhttps://www.mdpi.com/2223-7747/10/9/1914
ID Code:25648
Faculty:LTV - Fakulteten för landskapsarkitektur, trädgårds- och växtproduktionsvetenskap
Department:(LTJ, LTV) > Department of Plant Breeding (from 130101)
Deposited By: SLUpub Connector
Deposited On:12 Oct 2021 15:22
Metadata Last Modified:12 Oct 2021 15:31

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